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- 文献和实验
- 技术资料
- 保存条件:
-20℃&-80℃
- 保质期:
3个月~12个月
- 英文名:
pGL4.29[luc2P-CRE-Hygro]质粒
- 库存:
41
- 供应商:
上海禾午生物科技有限公司
- 规格:
2ug冻干粉&300ul甘油菌
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文献和实验Construction of Gene-Targeting Vectors by Recombineering
should be phenotypically normal unless they are bred to a Cre-expressing transgenic line. Depending on the nature of the promoter controlling Cre recombinase expression (ubiquitous or tissue-specific), deletion of the lox P-flanked ("floxed") region can occur
Developing Genetically Engineered Mouse Models to Study Tumor Suppression
step introduces three lox P sites (red ovals) and a neo‐tk gene by homologous recombination into the p 53 locus. Cre‐mediated deletion of neo and not deletion of the entire p 53 gene is possible when Cre is weakly expressed. The Trp53F2‐10 allele
越来越多的研究人员开始采用小分子干扰RNA(small interfering RNAs,siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链RNA分子, 能够以同源互补序列的mRNA为靶目标降解特定的mRNA,这个过程就是RNA干扰途径(RNA interference pathway)。RNAi的应用包括功能基因组学,药物靶筛选,细胞
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