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- 文献和实验
- 技术资料
- 英文名:
Qubit dsDNA HS Assay Kit Q32854
- 供应商:
研卉生物
- 规格:
500 assays
描述
The Qubit dsDNA HS (High Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.Which product to choose for dsDNA HS (High Sensitivity) quantitation?
• For 1–20 samples: use this Qubit dsDNA HS Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT dsDNA HS Assay Kit with microplate reader
Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.
3. Also available as a "1X" dsDNA assay kit that provides reagent and buffer in a formulation that is stable as a ready-to-use solution for up to one year after preparatio
规格
| Catalog # | Name | Size | 单价 (CNY) | Qty |
|---|---|---|---|---|
| Q10210 | Qubit™ RNA BR Assay Kit | 100 assays |
1,397.00
|
|
| Q10211 | Qubit™ RNA BR Assay Kit | 500 assays |
4,276.00
|
|
| Q32852 | Qubit™ RNA HS Assay Kit | 100 assays |
1,397.00
|
|
| Q32855 | Qubit™ RNA HS Assay Kit | 500 assays |
4,276.00
|
|
| Q32880 | Qubit™ microRNA Assay Kit | 100 assays |
1,411.00
|
|
| Q32881 | Qubit™ microRNA Assay Kit | 500 assays |
4,318.00
|
|
| Q32850 | Qubit™ dsDNA BR Assay Kit | 100 assays |
1,397.00
|
|
| Q32853 | Qubit™ dsDNA BR Assay Kit | 500 assays |
4,210.00
|
|
| Q32851 | Qubit™ dsDNA HS Assay Kit | 100 assays |
1,397.00
|
|
| Q32854 | Qubit™ dsDNA HS Assay Kit | 500 assays |
4,210.00
|
|
| Q10212 | Qubit™ ssDNA Assay Kit | 1 kit |
1,397.00
|
|
| Q33211 | Qubit™ Protein Assay Kit | 100 assays |
1,397.00
|
|
| Q33212 | Qubit™ Protein Assay Kit | 500 assays |
4,276.00
|
|
| Q32856 | Qubit™ Assay Tubes | 500 tubes |
1,207.00
|
| Sample Type (General): | dsDNA |
|---|---|
| For Use With (Equipment): | Qubit® Fluorometer |
| Quantitation Range: | 0.2-100 ng |
| Number of Reactions: | 500 reactions |
| Product Line: | Quant-iT™, Qubit™ |
| Product Size: | 500 assays |
| Green Features: | Fewer resources used and less waste |
| Shipping Condition: | Room Temperature |
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文献和实验RNAqueous® MAG-96 and then converted to cDNA. Target cDNA levels were analyzed in duplicate using a TaqMan Gene Expression Assay for survivin (Hs00153353_m1). Data shown here represent duplicate assays from single transfections. Survivin mRNA levels
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
plasmid (Addgene, Cambridge, MA, USA). 11. pUC19 plasmid (NEB). 2.3 Genome Modifi cation and Homologous Recombination Assay Reagents 1. 1× PBS. 2. QuickExtract? DNA extraction kit (Epicentre). 3. SURVEYOR Mutation Detection Kit
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