- ¥2980
- LMAI Bio
- LM-4730R-FITC
- 中国/美国/欧洲
- 2025年07月14日
- 详见说明书
- Human
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-4730R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-NADPH oxidase 4/FITC
- 抗体名：
Anti-NADPH oxidase 4/FITC
- 标记物：
FITC标记
- 宿主：
Human
- 适应物种：
Human
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
详见说明书
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-NADPH oxidase 4/FITC |
| 中文名称 | FITC标记的NADPH氧化酶4抗体 |
| 别 名 | Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX 1; KOX; Kox-1; NADPH; NADPH oxidase 4; Nox4; NOX4_HUMAN; Renal NAD(P)H-oxidase; RENOX. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 67kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NOX4 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response. Function: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. Subunit: Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein (Probable). Cell junction, focal adhesion (Probable). Nucleus (Probable). Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus. Tissue Specificity: Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord. Post-translational modifications: Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function. Similarity: Contains 1 FAD-binding FR-type domain. Contains 1 ferric oxidoreductase domain. Database links: Entrez Gene: 50507 Human Entrez Gene: 50490 Mouse Entrez Gene: 85431 Rat Omim: 605261 Human SwissProt: Q9NPH5 Human SwissProt: Q9JHI8 Mouse SwissProt: Q924V1 Rat Unigene: 371036 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 还原型辅酶烟酰胺腺嘌呤二核苷酸磷酸(NADPH)oxidase 4:还原型辅酶烟胺腺嘌呤二核苷酸（Nicotinamide adenine dinucleotide reduced, NADH）位于线粒体膜内，是细胞能量代谢所必需的辅酶，主要功能是经电子传递链产生ATP。NADH在维持细胞生长、分化和能量代谢中起重要的作用。 |
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文献和实验当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成； 方法与步骤： 根据Marshall氏法高效价的抗人球蛋白兔免疫血清，分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg，缓冲液为总量的10%； 2. 将以上溶液降温至4℃，按蛋白：荧光素=50—80mg：1mg的比例加入异硫氰酸荧光素，在0—4℃下电磁搅拌12—14h； 3.用半饱和硫酸铵将标记球蛋白
5. 过柱。取透析过夜的标记物，过葡萄糖凝胶G-25或G-50柱，分离出游离荧光素，收集标记的荧光抗体进行鉴定。
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