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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human p16INK4A C-terminus protein protein.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX57189
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
IHC-P
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
CDKN2A / p16INK4a
- 抗体英文名:
CDKN2A / p16INK4a antibody [IHC016]
- 抗体名:
CDKN2A / p16INK4a 抗体 [IHC016]
- 规格:
500 μl
IHC-P analysis of human colon tissue using GTX57189 CDKN2A / p16INK4a antibody [IHC016]
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文献和实验Immunohistochemistry (IHC) of Thymus Frozen Sections
a slide from PBS and wipe edges of each slide with KimWipe to create a static barrier. Add 175 µL of 1º antibody to each slide. Cover with a lid x1hr. Rinse by dipping in 2 staing jars containing PBS. Soak in a 3rd PBS jar x5 min. Add 175
IHC frozen sections staining protocol
, methanol or ethanol) at -20°C for 30 min. (Abcam recommends starting with acetone) 3. Fix with the pre cooled fixative for 5-10 min, at room temperature. 4. Rinse 3-4 X in PBS. 5. Continue with the immunohistochemical staining protocol
。所以,我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体:Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本:石蜡包埋人类肺部肿瘤样本煮沸设备:浴锅 (左),微波炉 (中),高压锅 (右)免疫染色—封闭在 IHC–P 中,我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品,时长 1 小时,以防止非特异性背景染色。在进行 IHC–F 时,在含有 0.3% Triton. X–100 的 1X
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