- ￥1700 - 4000
- GeneTex
- 美国
- GTX628996
- 2025年07月14日
- WB, ICC/IF
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX628996
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF
- 浓度：
1.05 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H2A.XS139ph (phospho Ser139)
- 抗体英文名：
Histone H2A.XS139ph (phospho Ser139) antibody [GT1021]
- 抗体名：
Histone H2A.XS139ph (phospho Ser139) 抗体 [GT1021]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] detects Histone H2A.XS139ph (phospho Ser139) protein at Nucleus by immunofluorescent analysis.
Sample: 10uM Cisplatin for 24 hrs HeLa cells were fixed in 4% PFA/PBS for 15 min.
Green: Histone H2A.XS139ph (phospho Ser139) protein stained by Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] (GTX628996) diluted at 1:500.
Blue: Hoechst 33342 staining.
Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] detects Histone H2A.XS139ph (phospho Ser139) protein by Western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract (untreated)
B. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 24hr)
15 % SDS-PAGE
Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] (GTX628996) dilution: 1:1000
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文献和实验Zhang P et al., Biochem Biophys Res Commun 2017 (PMID:28676400)
Lin CC et al., Cell Death Differ 2020 (PMID:31988496)
Chao-Chieh Lin et al., bioRxiv 2019 (Epub)
Histone H2AX Phosphorylation: A Marker for DNA Damage
) are generated that rapidly result in the phosphorylation of histone H2A variant H2AX. Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
Growth factor stimulation results in phosphorylation of histone H3 at ser 10 and this correlated with expression of immediate early genes suggesting that this phosphorylation is associated with transcriptional activation. Although Western
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