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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
嵘崴达
- 保质期:
见产品外包装
- 英文名:
Proteinase K (50 uL)
- 库存:
需确认
- 规格:
50 uL
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文献和实验Proteinase K is a serine protease and the main proteolytic enzyme produced by the fungus Tritirachium album Limber (1 ). The enzyme has abroad specificity, cleaving peptide bonds C-terminal to a number of amino acids. The enzyme is produced
RNA Extraction by the Proteinase K Method
. Next the polysomes are dissociated with SDS and proteinase K and finally the protein is removed by several phenol/choloroform extractions (1 ,2 ).
SQ Blood Mini Protocol for 50ul Clotted Blood
up an down a few times to mix. 3. Add 3 ul Proteinase K solution (20mg/ml) and mix by inverting 20 times. 4. Incubate at 55°C for 1 hour to overnight until clots has dissolved. 5. Place the tube on ice for 1 minute. 6. Add 3 ul RNase A to the cell
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