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- 详细信息
- 文献和实验
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- 保存条件:
Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C.
- 保质期:
参照说明书可保存1-2年
- 英文名:
AKT Peptide
- 库存:
一定量库存
- 供应商:
华拓生物
- 规格:
0.1 mg
| 产品名称: | AKT Peptide |
| 产品链接: | http://www.abbiotec.com/peptides/akt-peptide |
| 货号: | 350534 |
| 规格: | 0.1 mg |
| 产品描述: | RAC-alpha serine/threonine-protein kinase (AKT1) signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as PDGF, EGF, and IGF-I. This antibody may cross-react with AKT1, 2 and 3. |
| 序列: | The synthetic peptide used to raise the antibody Cat. No. 200172 is selected from a sequence within the C-term region of human AKT. For blocking experiments, a 10 to 100 fold molar excess to antibody is recommended. |
| 分子式: | C92H133N27O29S1 |
| 分子量: | 2113.2 g/mol |
| 纯度: | Purity > 80% by HPLC |
| 别名: | RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; Protein kinase B; PKB; C-AKT; AKT1; RAC |
| 形态: | Each vial contains 0.1 mg of lyophilized peptide. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml. Use at 5.6 ug/ml for a 100X excess over antibody for maximum blocking effect. |
| 溶解方法: | Distilled water for a solution up to 2 mg/ml, otherwise we recommend using acetonitrile. |
| 保存方法: | Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. |
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文献和实验yukituki 最近做Akt的WB遇到了问题: 我用的是CST的Akt抗体以及pAkt抗体,染小鼠组织(肝,肾,脾,肺,脑等)。 5%BSA(TBST稀释)4度封闭过夜 之后一抗是5%脱脂牛奶稀释,1:500,过夜,二抗1:2000稀释,2-3h 染出来的片子很脏,而且Akt勉强可以见到,但是pAkt没染出来条带 提组织用的是RIPA(每100mg用500ul),加了PMSF以及NaF,冰上
二个以上的α -氨基酸以肽键连接起来而形成的化合物。根据构成氨基酸的数目 2、 3、 4……叫做二肽( dipeptide)、三肽、四肽……。大体上由 10以下的肽键形成的肽总称为寡肽 ( Oligopeptide),由多数肽键构成的肽总称为多肽。每个肽按照构成它的氨基酸的名称,从具有游离α -氨基的末端氨基酸依次命名。例如 称为甘氨酰丙氨酸的二肽。蛋白质是由一条或几条长链状多肽构成的。象谷胱甘肽和肌肽那样的低
jixianxiu1982 各位大侠,有谁知道AKT1的基因序列呀,我找了好久没找到。 doctormy 人类AKT1序列: http://www.ncbi.nlm.nih.gov/nuccore/33875493 jixianxiu1982 谢谢doctormy大侠 jixianxiu1982 我还想继续知道其中PH结构域的基因序列,该怎么找
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