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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Human
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
5 - 120 ng/ml
- 供应商:
康朗生物
- 规格:
96T
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Mouse CEA / Carcinoembryonic Antigen ELISA Kit (Sandwich ELISA) - LS-F5042
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Human CEA / Carcinoembryonic Antigen ELISA Kit (Sandwich ELISA) - LS-F5206
Type: Quantitative Sandwich ELISA
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Human CEA / Carcinoembryonic Antigen ELISA Kit (Sandwich ELISA) - LS-F432
Type: Quantitative Sandwich ELISA
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Reactivity: Human
Range: 0.343-83.33 ng/ml
Detection: Colorimetric - 450nm (TMB)
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Product Description
LS-F9998 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CEA / Carcinoembryonic Antigen in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CEA / Carcinoembryonic Antigen as low as 2 nanograms per millilter.About CEA / Carcinoembryonic Antigen
Carcinoembryonic antigen (CEA) describes a set of highly related glycoproteins involved in cell adhesion. CEA is normally produced in gastrointestinal tissue during fetal development, but the production stops before birth. Therefore CEA is usually present only at very low levels in the blood of healthy adults. However, the serum levels are raised in some types of cancer, which means that it can be used as a tumor marker in clinical tests. Serum levels can also be elevated in heavy smokers.
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CEA / Carcinoembryonic Antigen
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human CEA. No significant cross-reactivity or interference between human CEA and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human CEA and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
5 - 120 ng/ml
Sensitivity
2 ng/ml
Precision
Intra-assay: CV%<10% Inter-assay: CV%<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Assay plate
- 6 x Standard
- HRP-conjugate
- Wash Buffer (20 x concentrate)
- Substrate A
- Substrate B
- Stop Solution
- Adhesive Strip (For 96 wells)
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for CEA. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CEA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Sandwich ELISA Platform Overview
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文献和实验thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
CD66a/CD66b/CD66c/CD66d/CD66e/CD66f/CD67分子
)和结构: gp54-72 功 能:可能与成功妊娠有关,保护胎儿免受母体免疫系统的损害 CD67 常用单克隆抗体或代号: 改为CD66b CD66a Aka CEACAM1, biliary glycoprotein, BGP, C-CAM Primordial protein of carcinoembryonic antigen (CEA) family
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