- $1015
- LSBio
- 0.156 - 10 ng/ml
- LS-F8573
- 美国
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
0.156 - 10 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Mouse IRS2 / IRS-2 ELISA Kit (Sandwich ELISA)
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Product Description
LS-F8573 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IRS2 / IRS-2 in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IRS2 / IRS-2 as low as 0.053 nanograms per millilter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IRS2 / IRS-2
Reactivity
Mouse
Specificity
Insulin Receptor Substrate 2 (IRS2) No significant cross-reactivity or interference between Insulin Receptor Substrate 2 (IRS2) and analogs was observed.
Manual
Intended Sample Types
Cell Lysates, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.053 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About IRS2 / IRS-2
Q9Y4H2 NM_003749 NP_003740.2
Popular IRS2 / IRS-2 Elisa Kits
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Human IRS2 / IRS-2 ELISA Kit (Custom ELISA) - LS-F14206
Reactivity: Human
Human IRS2 / IRS-2 ELISA Kit (Sandwich ELISA) - LS-F35115
Reactivity: Human
Range: 0.625-40 ng/ml
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Sandwich ELISA Platform Overview
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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