- $665
- LSBio
- To be determined
- LS-F23022
- 美国
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
Mouse IL1A / IL-1 Alpha ELISA Kit (Sandwich ELISA)
Product Description
LS-F23022 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IL1A / IL-1 Alpha in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IL1A / IL-1 Alpha as low as 9.375 picograms per milliliter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IL1A / IL-1 Alpha
Reactivity
Mouse
Specificity
This kit recognizes natural and recombinant Mouse IL-1a.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
15.625 - 1000 pg/ml
Sensitivity
9.375 pg/ml
Assay Time
3.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
- Prepare all reagents, samples and standards.
- Add 100 µl standard or sample to each well and incubate for 90 minutes at 37°C.
- Aspirate and add 100 µl prepared Detection Antibody and incubate of 1 hour at 37°C.
- Aspirate and wash 3 times.
- Add 100 µl HRP Conjugate and incubate for 30 minutes at 37°C.
- Aspirate and wash 5 times.
- Add 90 µl Substrate Solution and incubate for 15-20 minutes at 37°C.
- Add 50 µl Stop Solution.
- Read immediately at 450nm.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About IL1A / IL-1 Alpha
P01583 NM_000575 NP_000566.3
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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