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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
pHis2
- 保质期：
6个月
- 供应商：
上海钰博生物科技有限公司
- 保存条件：
-20℃
- 规格：
1ug/5ug
pHis2
| 编号 | 载体名称 |
| YB2110 | pHis2 |
pHis2载体基本信息
| 载体名称: | pHis2 |
|---|---|
| 质粒类型: | 酵母单杂交载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7207bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡纳霉素 |
| 筛选标记: | -- |
| 备注: | 含HIS3报告基因 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
pHis2载体质粒图谱和多克隆位点信息
pHis2载体序列
LOCUS pHIS-2 7207 bp DNA SYN DEFINITION pHIS-2 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7207 /organism="pHIS-2" /mol_type="other DNA" gene 152..811 /label="HIS3" /gene="HIS3" CDS 152..814 /label="ORF frame 2" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVA EQATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQ AFKEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMI PHFLESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGV LM*" terminator 815..1449 /label="HIS3_3UTR_terminator" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVA EQATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQ AFKEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMI PHFLESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGV LM*" CDS 1117..1716 /label="ORF frame 1" /translation="MAELSEQVQNLSINDNNENGYVPPHLRGKPRSARNNMSNYNNNN GGYNGGRGGGSFFSNNRRGGYGNGGFFGGNNGGSRSNGRSGGRWIDGKHVPAPRNEKA EIAIFGVPEDPLRRTHRGRHHRRHRCGCWRLYRRHHRWGRSGSPLRAHERLFRRGYGG RPRGRGTVGRHLLACTIPCGGGAQRPQPTTGLLPNAGVA*" CDS 1385..2350 /label="ORF frame 2" /translation="MANMSQLQETKRPRSPYLVSPRILYAGRIVAGITGATGAVAGAY IADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLG CFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIG PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*" gene 1460..2350 /label="tet (300 - 1190)" /gene="tet (300 - 1190)" /translation="MANMSQLQETKRPRSPYLVSPRILYAGRIVAGITGATGAVAGAY IADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLG CFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIG PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*" misc_feature complement(1512..1531) /label="pBRrevBam_primer" /translation="MANMSQLQETKRPRSPYLVSPRILYAGRIVAGITGATGAVAGAY IADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLG CFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIG PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*" CDS complement(1697..2155) /label="ORF frame 1" /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*" gene 2858..3532 /label="TRP1" /gene="TRP1" /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*" CDS 2858..3532 /label="ORF frame 2" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" promoter complement(3613..3632) /label="T3_promoter" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" promoter complement(3649..3667) /label="M13_reverse_primer" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" misc_feature complement(3666..3688) /label="M13_pUC_rev_primer" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" promoter complement(3702..3731) /label="lac_promoter" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" CDS complement(4814..5608) /label="ORF frame 1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene complement(4817..5596) /label="NeoR/KanR (variant)" /gene="NeoR/KanR (variant)" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" promoter complement(5650..5678) /label="AmpR_promoter" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" misc_feature complement(5741..6257) /label="CEN6_ARS4" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" misc_feature complement(6356..6378) /label="pGEX_3_primer" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" rep_origin complement(6696..7002) /label="f1_origin" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" CDS complement(6802..59) /label="ORF frame 3" /translation="MPGISRPRIDSRTRELPGNSPYSESYYNSLAVVLQRRDWENPGV TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVT ATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" misc_feature complement(7035..7183) /label="lacZ_a" /translation="MPGISRPRIDSRTRELPGNSPYSESYYNSLAVVLQRRDWENPGV TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVT ATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" misc_feature 7149..7171 /label="M13_pUC_fwd_primer" /translation="MPGISRPRIDSRTRELPGNSPYSESYYNSLAVVLQRRDWENPGV TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVT ATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" promoter 7164..7180 /label="M13_forward20_primer" /translation="MPGISRPRIDSRTRELPGNSPYSESYYNSLAVVLQRRDWENPGV TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVT ATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" promoter 7187..7205 /label="T7_promoter" /translation="MPGISRPRIDSRTRELPGNSPYSESYYNSLAVVLQRRDWENPGV TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVT ATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" ORIGIN 1 GAATTCCCGG GGAGCTCACG CGTTCGCGAA TCGATCCGCG GTCTAGAAAT TCCTGGCATT 61 ATCACATAAT GAATTATACA TTATATAAAG TAATGTGATT TCTTCGAAGA ATATACTAAA 121 AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC TAGTAAAGCG 181 TATTACAAAT GAAACCAAGA TTCAGATTGC GATCTCTTTA AAGGGTGGTC CCCTAGCGAT 241 AGAGCACTCG ATCTTCCCAG AAAAAGAGGC AGAAGCAGTA GCAGAACAGG CCACACAATC 301 GCAAGTGATT AACGTCCACA CAGGTATAGG GTTTCTGGAC CATATGATAC ATGCTCTGGC 361 CAAGCATTCC GGCTGGTCGC TAATCGTTGA GTGCATTGGT GACTTACACA TAGACGACCA 421 TCACACCACT GAAGACTGCG GGATTGCTCT CGGTCAAGCT TTTAAAGAGG CCCTAGGGGC 481 CGTGCGTGGA GTAAAAAGGT TTGGATCAGG ATTTGCGCCT TTGGATGAGG CACTTTCCAG 541 AGCGGTGGTA GATCTTTCGA ACAGGCCGTA CGCAGTTGTC GAACTTGGTT TGCAAAGGGA 601 GAAAGTAGGA GATCTCTCTT GCGAGATGAT CCCGCATTTT CTTGAAAGCT TTGCAGAGGC 661 TAGCAGAATT ACCCTCCACG TTGATTGTCT GCGAGGCAAG AATGATCATC ACCGTAGTGA 721 GAGTGCGTTC AAGGCTCTTG CGGTTGCCAT AAGAGAAGCC ACCTCGCCCA ATGGTACCAA 781 CGATGTTCCC TCCACCAAAG GTGTTCTTAT GTAGTGACAC CGATTATTTA AAGCTGCAGC 841 ATACGATATA TATACATGTG TATATATGTA TACCTATGAA TGTCAGTAAG TATGTATACG 901 AACAGTATGA TACTGAAGAT GACAAGGTAA TGCATCATTC TATACGTGTC ATTCTGAACG 961 AGGCGCGCTT TCCTTTTTTC TTTTTGCTTT TTCTTTTTTT TTCTCTTGAA CTCGAGAAAA 1021 AAAATATAAA AGAGATGGAG GAACGGGAAA AAGTTAGTTG TGGTGATAGG TGGCAAGTGG 1081 TATTCCGTAA GAACAACAAG AAAAGCATTT CATATTATGG CTGAACTGAG CGAACAAGTG 1141 CAAAATTTAA GCATCAACGA CAACAACGAG AATGGTTATG TTCCTCCTCA CTTAAGAGGA 1201 AAACCAAGAA GTGCCAGAAA TAACATGAGC AACTACAATA ACAACAACGG CGGCTACAAC 1261 GGTGGCCGTG GCGGTGGCAG CTTCTTTAGC AACAACCGTC GTGGTGGTTA CGGCAACGGT 1321 GGTTTCTTCG GTGGAAACAA CGGTGGCAGC AGATCTAACG GCCGTTCTGG TGGTAGATGG 1381 ATCGATGGCA AACATGTCCC AGCTCCAAGA AACGAAAAGG CCGAGATCGC CATATTTGGT 1441 GTCCCCGAGG ATCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC 1501 GGTTGCTGGC GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG 1561 GCTCATGAGC GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT 1621 GGGCGCCATC TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT 1681 ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAC CGATGCCCTT 1741 GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC 1801 ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTGGGT 1861 CATTTTCGGC GAGGACCGCT TTCGCTGGAG CGCGACGATG ATCGGCCTGT CGCTTGCGGT 1921 ATTCGGAATC TTGCACGCCC TCGCTCAAGC CTTCGTCACT GGTCCCGCCA CCAAACGTTT 1981 CGGCGAGAAG CAGGCCATTA TCGCCGGCAT GGCGGCCGAC GCGCTGGGCT ACGTCTTGCT 2041 GGCGTTCGCG ACGCGAGGCT GGATGGCCTT CCCCATTATG ATTCTTCTCG CTTCCGGCGG 2101 CATCGGGATG CCCGCGTTGC AGGCCATGCT GTCCAGGCAG GTAGATGACG ACCATCAGGG 2161 ACAGCTTCAA GGATCGCTCG CGGCTCTTAC CAGCCTAACT TCGATCATTG GACCGCTGAT 2221 CGTCACGGCG ATTTATGCCG CCTCGGCGAG CACATGGAAC GGGTTGGCAT GGATTGTAGG 2281 CGCCGCCCTA TACCTTGTCT GCCTCCCCGC GTTGCGTCGC GGTGCATGGA GCCGGGCCAC 2341 CTCGACCTGA ATGGAAGCCG GCGGCACCTC GCTAACGGAT TCACCACTCC AAGAATTGGA 2401 GCCAATCAAT TCTTGCGGAG AACTGTGAAT GCGCAAACCA ACCCTTGGCA GAACATATCC 2461 ATCGCGTCCG CCATCTCCAG CAGCCGCACG CGGCGCATCG GGGGGGGGGT TTCAATTCAA 2521 TTCATCATTT TTTTTTTATT CTTTTTTTTG ATTTCGGTTT CTTTGAAATT TTTTTAACGA 2581 CATTACTATA TATATAATAT AGGAAGCATT TAATAGAACA GCATCGTAAT ATATGTGTAC 2641 TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGAAGATATT CTTTATTGAA AAATAGCTTG 2701 TCACCTTACG TACAATCTTG ATCCGGAGCT TTTCTTTTTT TGCCGATTAA GAATTAATTC 2761 GGTCGAAAAA AGAAAAGGAG AGGGCCAAGA GGGAGGGCAT TGGTGACTAT TGAGCACGTG 2821 AGTATACGTG ATTAAGCACA CAAAGGCAGC TTGGAGTATG TCTGTTATTA ATTTCACAGG 2881 TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG CAGAATGTGC 2941 TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA AGAGAACAAT 3001 TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA ATAGTTCAGG 3061 CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG TTTTGGCTCT 3121 GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT GGCAAGAATA 3181 CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG ACTGCAACAT 3241 ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG ATTCAGAAGC 3301 AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG GAAGGCAAGA 3361 GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA 3421 TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG 3481 TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC 3541 TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCCT AGAGTCGACC TGCAGGCATG 3601 CAAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTTCGAGCTT GGCGTAATCA TGGTCATAGC 3661 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 3721 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 3781 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 3841 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 3901 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 3961 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 4021 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 4081 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 4141 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 4201 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 4261 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 4321 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 4381 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 4441 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG 4501 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 4561 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 4621 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 4681 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 4741 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 4801 CTTGGTCTGA CAGTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT 4861 CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT 4921 CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG GTCCGCCACA CCCAGCCGGC 4981 CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT 5041 CGTCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA 5101 GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG 5161 CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG 5221 TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG 5281 CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT 5341 CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA 5401 GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT 5461 TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC 5521 CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC 5581 CTGCGTGCAA TCCATCTTGT TCAATCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 5641 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 5701 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGC GGACGGATCG CTTGCCTGTA 5761 ACTTACACGC GCCTCGTATC TTTTAATGAT GGAATAATTT GGGAATTTAC TCTGTGTTTA 5821 TTTATTTTTA TGTTTTGTAT TTGGATTTTA GAAAGTAAAT AAAGAGTAGA AGAGTTACGG 5881 AATGAAGAAA AAAAAATAAA CAAAGGTTTA AAAAATTTCA ACAAAAAGCG TACTTTACAT 5941 ATATATTTAT TAGACAAGAA AAGCAGATTA AATAGATATA CATTCGATTA ACGATAAGTA 6001 AAATGTAAAA TCACAGGATT TTCGTGTGTG GTCTTCTACA CAGACAAGAT GAAACAATTC 6061 GGCATTAATA CCTGAGAGCA GGAAGAGCAA GATAAAAGGN AGTATTTGTT GGCGATCCCC 6121 CTAGAGTCTT TTACATCTTC GGAAAACAAA AACTATTTTT TCTTTAATTT CTTTTTTTAC 6181 TTTCTATTTT TAATTTATAT ATTTATATTA AAAAATTTAA ATTATAATTA TTTTTATAGC 6241 ACGTGATGAA AAGGACCGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA 6301 ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT 6361 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC 6421 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG 6481 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG 6541 TAAGGAGAAA ATACCGCATC AGGAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT 6601 AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA 6661 TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC 6721 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG 6781 CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT 6841 AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT 6901 GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC 6961 GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC 7021 GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC 7081 GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC 7141 AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTGTAAT ACGACTCACT 7201 ATAGGGC
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文献和实验相关实验
-线性化)、5 μg pHIS2-PBE1钓饵载体、20 μL Herring Testes Carrier DNA(用前于100 ℃变性5 min，然后迅速置于冰上)、600 μL 酵母Y187感受态细胞，轻轻混匀，再加入2.5 mL PEG/LiAc (40% PEG 3350，1×TE Buffer，0.1 mol/L LiAc)，轻轻混匀。30℃温育45 min，每隔15 min混合细胞1次，然后加入160 μL DMSO，混匀后置42℃热冲击20 min，每隔10
的功能蛋白编码基因，验证反式转录调控因子的DNA结合结构域，准确定位参与特定蛋白质结合的核苷酸序列。 酵母单杂交用的cDNA文库用什么试剂盒构建? 单杂交文库可以用酵母双杂交试剂盒中的SMART试剂盒来构建 用BD公司的酵母单杂交文库构建和筛选试剂盒进行酵母单杂交文库的构建。具体操作步骤为：在15 mL试管中依次加入如下成分：20 μL SMART cDNA、6 μL pGADT7-Rec2(0.5 μg/μL，SmaI-线性化)、5 μg pHIS2-PBE
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