- ￥5354
- Invitrogen
- MA1-135
- 美国
- 2025年10月15日
- WB，1:250-1:1,000
- Tag
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海研卉生物科技
- 库存：
1周
- 靶点：
6x His Tag抗体
- 级别：
4E3D10H2/E3科研
- 目录编号：
MA1-135
- 克隆性：
单克隆
- 抗原来源：
Mixture of N-terminal and C-terminal 6x-His peptides, CG-HHHHHH and HHHHHH-GC
- 抗体英文名：
6x-His Tag Monoclonal Antibody (4E3D10H2/E3)
- 抗体名：
6x His Tag抗体
- 标记物：
无
- 适应物种：
Tag
- 亚型：
Mouse / IgG1
- 应用范围：
WB，1:250-1:1,000
- 浓度：
1 mg/mL
- 规格：
48个/盒，10盒/箱
6x His Tag抗体
6x-His Tag Monoclonal Antibody (4E3D10H2/E3)
产品信息
应用
建议稀释比
已发表文章
免疫印迹 (WB)
1:250-1:1,000
查看19篇已发表文章
免疫组化 (IHC)
-
-
免疫组化（石蜡） (IHC (P))
-
查看1篇已发表文章
免疫细胞化学 (ICC/IF)
5 µg/mL
查看1篇已发表文章
流式细胞分析 (Flow)
-
查看2篇已发表文章
酶联免疫吸附实验 (ELISA)
25-500 ng/mL
查看5篇已发表文章
免疫沉淀 (IP)
2 µg
查看5篇已发表文章
点杂交 (DB)
-
查看1篇已发表文章
亲和纯化 (AP)
-
查看2篇已发表文章
其他PubMed (Misc)
-
查看2篇已发表文章
产品规格
种属反应
Tag
已发表种属
C. elegans, Tag
宿主/亚型
Mouse / IgG1
分类
Monoclonal
类型
Antibody
克隆号
4E3D10H2/E3
抗原
Mixture of N-terminal and C-terminal 6x-His peptides, CG-HHHHHH and HHHHHH-GC
偶联物
Unconjugated
形式
浓度
纯化类型
保存液
内含物
保存条件
运输条件
产品详细信息
By Western blot, MA1-135 detects both N-terminal and C-terminal His-tagged proteins.
靶标信息
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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文献和实验Protein Expression and Purification of RPA70-AB (181-422)
),and a gradient program running for 2 column size using Buffer A and Buffer B (same as Buffer A but containing 0.3 M Imidazole).13.Pool fractions after checking them on SDS-PAGE.TEV Protease CleavageComment: TEV protease has a 6x His-tag on its NT
Genotyping using Affymetrix arrays
adaptor (5’-ATT ATG AGC ACG ACA GAC GCC TGA TCT-3’ and 5’phosphate AGC TAG ATC AGG CGT CTG TCG TGC TCA TAA-3’) (Affymetrix) are ligated to the digested DNAs with T4 DNA Ligase (New England BioLabs) in 25µL for 2 hours at 16°C. The ligations are stopped
蛋白表达系统在表达膜蛋白、有毒蛋白、二硫键蛋白、抗体片段等方面具有独特优势。由于可以使用线性模板，利用PCR方法就可以轻松实现在目的基因两侧加入需要的转录和翻译所需的因子，不需要繁琐的载体构建过程。线性模板的使用还可以实现蛋白的高通量合成和筛选。 ALiCE® Kit无细胞蛋白质表达试剂盒 (植物细胞裂解液) (Sigma货号：AL0103000) ALiCE®无细胞蛋白质表达试剂盒是一种独创的真核无细胞系统，可利用植物细胞裂解物在批量处理模式下将蛋白质产量提升到前所未有的3mg/mL。ALiCE®
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