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- 文献和实验
- 技术资料
- 英文名：
FastDigest Psp1406I
- 供应商：
上海研卉生物
- 库存：
大量
- 规格：
20 reactions
描述
| 5' | A | A ↓ | C | G | T | T | 3' | ||||
| 3' | T | T | G | C ↑ | A | A | 5' |
Thermo Scientific FastDigest Psp1406I restriction enzyme recognizes AA^CGTT site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: AclI, UbaN9I.
Thermo Scientific FastDigest Psp1406I is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
规格
| Compatible Buffer: | 10x FastDigest Buffer/FastDigest Green Buffer |
|---|---|
| Enzyme: | Psp1406I |
| Methylation Sensitivity: | CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive |
| Optimal Reaction Temperature: | 37° C |
| Product Line: | FastDigest |
| Product Size: | 20 reactions |
| Sensitive to Heat Inactivation: | Yes |
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文献和实验近端肾小管功能检查： 近端肾小管功能检查是酚红排泄率可作为判断近端小管排泄功能的粗略指标。由于各种原因引起的肾血流量下降或尿路梗阻，均会造成PSP排泄量减低，故PSP排泄量实际上是一项肾血流量和近端肾小管的简易评价试验，该试验由于方法学不灵敏，目前多数医院已经淘汰医学`教育网搜集整理。迄今为止尚没有一个令人满意的近端肾小管功能的试验。
药动学实验 药动学研究药物在生物体内的转运、代谢变化过程和药物浓度随时间变化的规律。其基本的过程是药物的吸收、分布、代谢、排泄。本实验包括三部分内容： 1．通过测定血、肝、脂肪组织中磺胺类药物浓度，以了解药物的吸收及分布； 2．通过测定正常及肾功能受损家兔血浆中PSP(酚磺酞)浓度分别求得PSP的血浆半衰期，以了解肾功
Sporulation in Liquid Media 1) Grow cells in 25mls of PSP2 (in 250ml flask) supplemented with 25% of the recommended concentration of amino acids until they are about 1x107 cells/ml (~1 day). 2) Wash with once with 25mls
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