Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donor’s apheresis (Blood Bank, National Institute of Health, Bethesda, MD) using lymphocyte separation medium (MPbio, Solon, OH, USA) as previously described .. CD14+ monocytes were isolated from PBMCs using MACS ...More
Rapid Response to the Combination of Lenvatinib and Pembrolizumab in Patients with Advanced Carcinomas (Lung Adenocarcinoma and Malignant Pleural Mesothelioma)
Isolation of peripheral blood mononuclear cells (PBMCs) from the peripheral blood of the patients was carried out according to a standard protocol using lymphocyte separation medium (MP Biomedicals, Irvine, CA, SKU 0850494-CF).. Then, PBMCs were cultured with 200U IL-2, and two days later, CD8+ T cells were isolated by using human CD8 Microbeads (Miltenyi Biotec, Bergisch Gladbach, North Rhine-Westphalia, 130-045-201, Bergisch Gladbach, Germany), LS column (Miltenyi Biotec, Bergisch Gladbach, North Rhine-Westphalia, 130-042-401), and MidiMACS Separator (130-042-302).Less
产品用途:
从去纤维蛋白或肝素化的全血中分离淋巴细胞
产品特点:
不同于应用传统Boyum配方制得的淋巴细胞提取液,MP Biomedicals出品的淋巴细胞提取液(Lymphocyte Separation Medium - LSM™),成功地运用泛影钠代替了甲泛影钠。