Hexokinase已糖激酶

Hexokinase已糖激酶
产品编号 H4502 | CAS号 9001-51-8 | Sigma
Hexokinase from Saccharomyces cerevisiae

Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)
别名: ATP:D-Hexose-6-phosphotransferase Hexokinase from yeast
Reconstitution

Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days.
Unit Definition

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.
Physical form

Lyophilized powder containing approx. 15% sodium citrate
Biochem/physiol Actions

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
type Type F-300
form lyophilized powder
purified by crystallization
anion traces sulfate (SO42-): free
foreign activity ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%
上海研卉生物科技有限公司 ，是一家专注于生命科学和生物技术领域的高科技企业，专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司主营业务： 试剂盒：酶联免疫试剂盒、免疫印迹试剂盒、免疫组化试剂盒、细胞凋亡检测试剂盒、分子生物学试剂盒。 细胞：原装进口细胞、国产肿瘤细胞、国产正常细胞。 耗材：细胞培养耗材、普通实验耗材。 生化试剂：原装进口生化试剂、进口分装生化试剂、国产生化试剂。 抗体：免疫组化、免疫荧光、流式细胞检测、免疫细胞化学。