DTT

DTT

1,4-Dithiothreitol

别名: 1,4-二巯基苏糖醇, 1,4-二硫苏糖醇, DL-二硫苏糖醇, DTT, cleland′s 试剂, 二硫苏糖醇，1,4-, 克莱兰氏试剂

General description

1,4-dithiothreitol is commonly known as Cleland′s reagent. It confers protection to thiol groups and reduces disulfide bonds in peptides and proteins. It reduces disulfide bonds to sulfhydryl group and is commonly used in receptor studies, to determine the functional importance of disulfide bonds during receptor occupancy and functionality. It also maintains monothiols completely in the reduced state.

Application

1,4-二硫苏糖醇可用于：
• 蛋白和酶的分离、纯化和表征
• 酶活的测量（酶的重激活）
• 测定蛋白和酶中的二硫基团
• 扩增前的DNA提取

Specifications

氧化形式：<2.5% （283nm吸光值）

Sequence

对映异构体
我们的DTT制剂是无光学活性的，例如它是D,L-DTT。

Preparation Note

储存条件（工作液）：在2到8 °C、容器密封且溶液通过氩气或氮气而免受大气中氧气影响时，DTT溶液可在Hepes缓冲液，pH 7.55中稳定一周。