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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
FastDigest Eco147I
- 库存:
大量
- 供应商:
上海研卉生物科技有限公司
- 规格:
100 reactions
描述
| 5' | A | G | G ↓ | C | C | T | 3' | ||||
| 3' | T | C | C ↑ | G | G | A | 5' |
Thermo Scientific FastDigest Eco147I restriction enzyme recognizes AGG^CCT site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: StuI, AatI, PceI, SseBI.
Thermo Scientific FastDigest Eco147I is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
规格
| Compatible Buffer: | 10x FastDigest Buffer/FastDigest Green Buffer |
|---|---|
| Enzyme: | Eco147I |
| Methylation Sensitivity: | Not CpG methylation-sensitive, Not dcm methylation-sensitive, dcm methylation-sensitive |
| Optimal Reaction Temperature: | 37° C |
| Product Line: | FastDigest |
| Product Size: | 100 reactions |
| Sensitive to Heat Inactivation: | Yes |
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文献和实验人 CD147 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 CD147 的 含量。 实验原理 : 本试剂盒应用 双抗体 夹心法测定 标本 中 人 CD147 水平。用纯化的 人 CD147 抗体 包被微孔板,制成固相 抗 体,往包被 单抗 的微孔中依次加入 CD147 ,再与HRP 标记的 CD147 抗体结合,形成抗体- 抗原 - 酶标
兔子 CD147 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定兔子血清,血浆及相关液体样本中 CD147 的 含量。 实验原理 : 本试剂盒应用 双抗体 夹心法测定 标本 中 兔子 CD147 水平。用纯化的 兔子 CD147 抗体 包被微孔板,制成固相 抗 体,往包被 单抗 的微孔中依次加入 CD147 ,再与HRP 标记的 CD147 抗体结合,形成抗体- 抗原
approaches that can be broadly divided into interactions arising from secreted diffusible factors and interactions through direct cell–cell contact. Here we describe a method for the study of direct ESI through CD147, an adhesion molecule present
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