- ¥968
- fermentas
- FD1794
- 2025年10月09日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 英文名：
FastDigest SsiI
- 供应商：
上海研卉生物科技有限公司
- 规格：
20 reactions
描述
| 5' | C ↓ | C | G | C | 3' | ||||
| 3' | G | G | C ↑ | G | 5' |
Thermo Scientific FastDigest SsiI restriction enzyme recognizes CCGC(-3/-1)^ site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: AciI, BspACI.
Thermo Scientific FastDigest SsiI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
规格
| Compatible Buffer: | 10x FastDigest Buffer/FastDigest Green Buffer |
|---|---|
| Enzyme: | SsiI |
| Methylation Sensitivity: | CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive |
| Optimal Reaction Temperature: | 37° C |
| Product Line: | FastDigest |
| Product Size: | 20 reactions |
| Sensitive to Heat Inactivation: | Yes |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验II (SSII) reverse transcriptase (Life Technologies) 2. Method The protocol below may be used for amplifying individual transcripts from RNA recovered from microdissected Cell populations as described in RNA-based
E.coli Total RNA Labeling Protocol for Spotted Microarray
Total 17ul To the RNA /hexamer mix add 17.0 ml of labeling mix and incubate 10min at RT Add 1.5 ml of appropriate CyDye dUTP (1mM stock) followed by 1.5 ml SSII reverse
E.coli Total RNA Labeling Protocol for Spotted Microarray
Add 1.5 ml of appropriate CyDye dUTP (1mM stock) followed by 1.5 ml SSII reverse transcriptase, mix well by tapping and pulse spin Incubate 1hr at 42 o C in the dark Add
技术资料暂无技术资料 索取技术资料
