BD流式破膜试剂

BD流式破膜试剂

货号	用途	产品名称	规格	备注
554655	针对胞内细胞因子	BD Cytofix™ Fixation Buffer	100ml	仅固定
554722		BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution	125ml	固定剂
554723		BD Perm/Wash™ Perm/Wash Buffer	100ml	破膜剂
554714		BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit	250T	含固定剂和破膜成分
554724		BD GolgiStop™ Protein Transport Inhibitor (Containing Monensin)	0.7ml	高尔基体转运蛋白抑制剂Monensin
555029		BD GolgiPlug™ Protein Transport Inhibitor (Containing Brefeldin A)	1ml	高尔基体转运蛋白抑制剂BFA
554715		BD Cytofix/Cytoperm™ Plus Fixation/Permeabilization Solution Kit with BD GolgiStop™ Monensin	250T	含固定剂+破膜剂+高尔基体转运蛋白抑制剂
555028		BD Cytofix/Cytoperm™ Plus Fixation/Permeabilization Solution Kit with BD GolgiPlug™ Brefeldin A	250T
GAS003		FIX & PERM™ Cell Permeabilization Kit	50T	固定剂+破膜剂
GAS004		FIX & PERM™ Cell Permeabilization Kit	200T
557885	PhosFlow 破膜试剂	BD Phosflow™ Perm/Wash Buffer I	125ml	破膜强度最弱，根据一抗的说明书来推荐
558052		BD Phosflow™ Perm Buffer II	125ml	破膜强度中等，根据一抗的说明书来推荐
558050		BD Phosflow™ Perm Buffer III	125ml	破膜程度最剧烈，根据一抗的说明书来推荐
558049	PhosFlow 固定剂	BD Phosflow™ Lyse/Fix Buffer 5X	250ml	固定剂（含裂红，针对含红细胞样本）
557870		BD Phosflow™ Fix Buffer I	250ml	固定剂
00-5523-00	针对核内转录因子	Foxp3 / Transcription Factor Staining Buffer Set	kit	针对FOXP3染色
562574		BD Pharmingen™ Transcription Factor Buffer Set	100T	针对核内的转录因子，比如FOXP3等

Description

BD Phosflow™ Perm/Wash Buffer I is intended to be used for the intracellular staining of post-translationally modified signaling proteins. BD Phosflow™ Perm/Wash Buffer I is used to permeabilize cells and to serve as an antibody diluent and cell wash buffer. It is optimized for use with the BD Phosflow™ brand of intracellular phosphorylated signaling protein-specific antibodies. BD Phosflow™ Perm/Wash Buffer I is provided as a 10X concentrated solution containing FBS and saponin. The presence of a small amount of precipitate may be observable and will not affect the performance of the buffer. Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining.

货号	用途	产品名称	规格
340345	清洗液	BD FACS™ Clean Solution	5L
334224	关机液	BD FACS™ Shutdown Solution	5L
342003	鞘液	BD FACSFlow™ Sheath Fluid	20L

货号	用途	产品名称	规格	备注
552843	补偿微球	BD™ CompBead Anti-Mouse Ig, κ/Negative Control Compensation Particles Set	6ml	要求一抗来源是mouse igg kappa链
552844		BD™ CompBead Anti-Rat Ig, κ/Negative Control Compensation Particles Set	6ml	要求一抗来源是rat igg kappa链
552845		BD™ CompBead Anti-Rat and Anti-Hamster Ig κ /Negative Control Compensation Particles Set	6ml	要求一抗来源是Hamster和rat kappa链
01-2222-42		UltraComp eBeads™ Compensation Beads	100T	通用型补偿微球

货号	用途	产品名称	规格
555899	裂红	BD Pharm Lyse™ Lysing Buffer 10X	100ml
00-4300-54		10X RBC Lysis Buffer (Multi-species)	50ml
00-4333-57		1X RBC Lysis Buffer（小鼠造血组织和人类外周血）	200ml
349202		BD FACS™ Lysing Solution 10X Concentrate	100ml
554656	抗体染色用	Stain Buffer (FBS)	500ml
554657		Stain Buffer (BSA)	500ml
00-4222-57		Flow Cytometry Staining Buffer	200ml
00-4222-26		Flow Cytometry Staining Buffer	600ml
561527	贴壁细胞消化	BD™ Accutase™ Cell Detachment Solution	100ml
564219	FCR阻断剂	Human BD Fc Block	50ug
564420		Human BD Fc Block	250ug
553141		Mouse BD Fc Block	0.1mg
553142		Mouse BD Fc Block	0.5mg
550270		Rat BD Fc Block	0.1mg
130-059-901		Fcr Blocing reagent，human	2ml
130-092-575		Fcr Blocing reagent，Mouse	100T
130-095-929	肿瘤组织处理为单细胞悬液	Tumor Dissociation Kit, human	25T
130-096-730		Tumor Dissociation Kit, mouse	50T

货号	用途	产品名称	规格
550583	刺激剂	Leukocyte Activation Cocktail, with BD GolgiPlug	200ul
555329	T细胞刺激扩增	Purified NA/LE Mouse Anti-Human CD3	0.5mg
555725		Purified NA/LE Mouse Anti-Human CD28	0.5mg
553057		Purified NA/LE Hamster Anti-Mouse CD3e	0.5mg
553294		Purified NA/LE Hamster Anti-Mouse CD28	0.5mg
130-091-441		T Cell Activation/Expansion Kit, human	kit
130-111-160		T Cell TransAct, human	2X2ml

货号	用途	产品名称	规格
653144	仪器校准	BD Accuri™ Spherotech 8-Peak Validation Beads (FL1 - FL3)	4ml
653145		BD Accuri™ Spherotech 6-Peak Validation Beads (FL4)	8ml
660584	C6鞘液添加剂	BD™ Sheath Additive	each
660634	C6-2月维护套装	BD Accuri™ C6 Plus 2-month Maintenance Kit	kit
661331	C6全年维护套装	BD Accuri™ C6 Plus 1-year Maintenance Kit	kit
660322	滤器	Sheath, Detergent, FACSclean Solution Bottle Filters	each
660586	流动室深度清洗试剂	BD™ Extended Flow Cell Clean Solution	8ml
660585	洗液	BD™ Detergent Solution Concentrate	each
340345	流式仪器清洗液	BD FACS™ Clean Solution	5L
653154	净化浓缩溶液	BD Accuri™ Decontamination Concentrate Solution	200ml
653156	抑菌剂浓缩溶液	BD Accuri™ Bacteriostatic Concentrate Solution	10L
653157	清洗浓缩溶液	BD Accuri™ Cleaning Solution Concentrate	1L
653158	C6流式细胞仪液流试剂盒	BD Accuri™ C6 Flow Cytometer Fluid Kit	kit
653160	BD Accuri™ C6 起始试剂盒	BD Accuri™ C6 Flow Cytometer Starter Kit	kit
661415	BD Accuri™ C6 Plus校准微球	CS&T RUO Beads	150T
661414		CS&T RUO Beads	50T