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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Perm/Wash Buffer I
- 保存条件：
低温
- 规格：
125 mL
| 货号 | 用途 | 产品名称 | 规格 | 备注 |
| 554655 | 针对胞内细胞因子 | BD Cytofix™ Fixation Buffer | 100ml | 仅固定 |
| 554722 | BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution | 125ml | 固定剂 | |
| 554723 | BD Perm/Wash™ Perm/Wash Buffer | 100ml | 破膜剂 | |
| 554714 | BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit | 250T | 含固定剂和破膜成分 | |
| 554724 | BD GolgiStop™ Protein Transport Inhibitor (Containing Monensin) | 0.7ml | 高尔基体转运蛋白抑制剂Monensin | |
| 555029 | BD GolgiPlug™ Protein Transport Inhibitor (Containing Brefeldin A) | 1ml | 高尔基体转运蛋白抑制剂BFA | |
| 554715 | BD Cytofix/Cytoperm™ Plus Fixation/Permeabilization Solution Kit with BD GolgiStop™ Monensin | 250T | 含固定剂+破膜剂+高尔基体转运蛋白抑制剂 | |
| 555028 | BD Cytofix/Cytoperm™ Plus Fixation/Permeabilization Solution Kit with BD GolgiPlug™ Brefeldin A | 250T | ||
| GAS003 | FIX & PERM™ Cell Permeabilization Kit | 50T | 固定剂+破膜剂 | |
| GAS004 | FIX & PERM™ Cell Permeabilization Kit | 200T | ||
| 557885 | PhosFlow 破膜试剂 | BD Phosflow™ Perm/Wash Buffer I | 125ml | 破膜强度最弱，根据一抗的说明书来推荐 |
| 558052 | BD Phosflow™ Perm Buffer II | 125ml | 破膜强度中等，根据一抗的说明书来推荐 | |
| 558050 | BD Phosflow™ Perm Buffer III | 125ml | 破膜程度最剧烈，根据一抗的说明书来推荐 | |
| 558049 | PhosFlow 固定剂 | BD Phosflow™ Lyse/Fix Buffer 5X | 250ml | 固定剂（含裂红，针对含红细胞样本） |
| 557870 | BD Phosflow™ Fix Buffer I | 250ml | 固定剂 | |
| 00-5523-00 | 针对核内转录因子 | Foxp3 / Transcription Factor Staining Buffer Set | kit | 针对FOXP3染色 |
| 562574 | BD Pharmingen™ Transcription Factor Buffer Set | 100T | 针对核内的转录因子，比如FOXP3等 |
Description
BD Phosflow™ Perm/Wash Buffer I is intended to be used for the intracellular staining of post-translationally modified signaling proteins. BD Phosflow™ Perm/Wash Buffer I is used to permeabilize cells and to serve as an antibody diluent and cell wash buffer. It is optimized for use with the BD Phosflow™ brand of intracellular phosphorylated signaling protein-specific antibodies. BD Phosflow™ Perm/Wash Buffer I is provided as a 10X concentrated solution containing FBS and saponin. The presence of a small amount of precipitate may be observable and will not affect the performance of the buffer. Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining.
| 货号 | 用途 | 产品名称 | 规格 |
| 340345 | 清洗液 | BD FACS™ Clean Solution | 5L |
| 334224 | 关机液 | BD FACS™ Shutdown Solution | 5L |
| 342003 | 鞘液 | BD FACSFlow™ Sheath Fluid | 20L |
| 货号 | 用途 | 产品名称 | 规格 | 备注 |
| 552843 | 补偿微球 | BD™ CompBead Anti-Mouse Ig, κ/Negative Control Compensation Particles Set | 6ml | 要求一抗来源是mouse igg kappa链 |
| 552844 | BD™ CompBead Anti-Rat Ig, κ/Negative Control Compensation Particles Set | 6ml | 要求一抗来源是rat igg kappa链 | |
| 552845 | BD™ CompBead Anti-Rat and Anti-Hamster Ig κ /Negative Control Compensation Particles Set | 6ml | 要求一抗来源是Hamster和rat kappa链 | |
| 01-2222-42 | UltraComp eBeads™ Compensation Beads | 100T | 通用型补偿微球 |
| 货号 | 用途 | 产品名称 | 规格 |
| 555899 | 裂红 | BD Pharm Lyse™ Lysing Buffer 10X | 100ml |
| 00-4300-54 | 10X RBC Lysis Buffer (Multi-species) | 50ml | |
| 00-4333-57 | 1X RBC Lysis Buffer（小鼠造血组织和人类外周血） | 200ml | |
| 349202 | BD FACS™ Lysing Solution 10X Concentrate | 100ml | |
| 554656 | 抗体染色用 | Stain Buffer (FBS) | 500ml |
| 554657 | Stain Buffer (BSA) | 500ml | |
| 00-4222-57 | Flow Cytometry Staining Buffer | 200ml | |
| 00-4222-26 | Flow Cytometry Staining Buffer | 600ml | |
| 561527 | 贴壁细胞消化 | BD™ Accutase™ Cell Detachment Solution | 100ml |
| 564219 | FCR阻断剂 | Human BD Fc Block | 50ug |
| 564420 | Human BD Fc Block | 250ug | |
| 553141 | Mouse BD Fc Block | 0.1mg | |
| 553142 | Mouse BD Fc Block | 0.5mg | |
| 550270 | Rat BD Fc Block | 0.1mg | |
| 130-059-901 | Fcr Blocing reagent，human | 2ml | |
| 130-092-575 | Fcr Blocing reagent，Mouse | 100T | |
| 130-095-929 | 肿瘤组织处理为单细胞悬液 | Tumor Dissociation Kit, human | 25T |
| 130-096-730 | Tumor Dissociation Kit, mouse | 50T |
| 货号 | 用途 | 产品名称 | 规格 |
| 550583 | 刺激剂 | Leukocyte Activation Cocktail, with BD GolgiPlug | 200ul |
| 555329 | T细胞刺激扩增 | Purified NA/LE Mouse Anti-Human CD3 | 0.5mg |
| 555725 | Purified NA/LE Mouse Anti-Human CD28 | 0.5mg | |
| 553057 | Purified NA/LE Hamster Anti-Mouse CD3e | 0.5mg | |
| 553294 | Purified NA/LE Hamster Anti-Mouse CD28 | 0.5mg | |
| 130-091-441 | T Cell Activation/Expansion Kit, human | kit | |
| 130-111-160 | T Cell TransAct, human | 2X2ml |
| 货号 | 用途 | 产品名称 | 规格 |
| 653144 | 仪器校准 | BD Accuri™ Spherotech 8-Peak Validation Beads (FL1 - FL3) | 4ml |
| 653145 | BD Accuri™ Spherotech 6-Peak Validation Beads (FL4) | 8ml | |
| 660584 | C6鞘液添加剂 | BD™ Sheath Additive | each |
| 660634 | C6-2月维护套装 | BD Accuri™ C6 Plus 2-month Maintenance Kit | kit |
| 661331 | C6全年维护套装 | BD Accuri™ C6 Plus 1-year Maintenance Kit | kit |
| 660322 | 滤器 | Sheath, Detergent, FACSclean Solution Bottle Filters | each |
| 660586 | 流动室深度清洗试剂 | BD™ Extended Flow Cell Clean Solution | 8ml |
| 660585 | 洗液 | BD™ Detergent Solution Concentrate | each |
| 340345 | 流式仪器清洗液 | BD FACS™ Clean Solution | 5L |
| 653154 | 净化浓缩溶液 | BD Accuri™ Decontamination Concentrate Solution | 200ml |
| 653156 | 抑菌剂浓缩溶液 | BD Accuri™ Bacteriostatic Concentrate Solution | 10L |
| 653157 | 清洗浓缩溶液 | BD Accuri™ Cleaning Solution Concentrate | 1L |
| 653158 | C6流式细胞仪液流试剂盒 | BD Accuri™ C6 Flow Cytometer Fluid Kit | kit |
| 653160 | BD Accuri™ C6 起始试剂盒 | BD Accuri™ C6 Flow Cytometer Starter Kit | kit |
| 661415 | BD Accuri™ C6 Plus校准微球 | CS&T RUO Beads | 150T |
| 661414 | CS&T RUO Beads | 50T |
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