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- 52904
- 2026年01月07日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
病毒RNA小提试剂盒
- 供应商：
QIAamp Viral RNA Mini Kit (50)
- 规格：
50T
Qiagen 52904 病毒RNA小提试剂盒
QIAamp病毒RNA试剂盒简化了从无细胞体液中纯化病毒RNA的过程，提供快速旋转柱、真空或平板离心程序，或在QIAcube上实现自动化。样本类型包括：
血浆与血清
脑脊液
尿
其他无细胞体液
细胞培养上清液
拭子
优化的缓冲液和酶可以裂解样品，稳定核酸，增强QIAamp膜对RNA的选择性吸附。为了保证RNA的完整性，在高度变性条件下裂解样品以灭活RNA酶。加入酒精，将裂解物装载到QIAamp旋转柱或96孔板上。洗涤缓冲液用于去除杂质，然后将纯的即用型RNA在水中或低盐缓冲液中洗脱。
真空处理
为了提高RNA纯化的速度和便利性，可以通过真空而不是离心来处理样品。QIAamp Mini旋转柱使用QIAamp Vac附件套件中提供的Vac阀和Vac连接器安装在QIAvac 24歧管上。如果样品流速差异很大，应使用真空阀，以确保真空的一致性。一次性真空连接器用于避免任何交叉污染。
QIAcube Connect上的自动处理
专用的QIAamp病毒RNA QIAcube试剂盒可在QIAcube Connect上实现自动化处理。此外，QIAamp病毒RNA迷你试剂盒可在QIAcube Connect上自动使用。QIAamp病毒RNA迷你配件套件提供了使用QIAcube Connect和QIAamp RNA迷你试剂盒进行自动化、低通量样品制备所需的额外缓冲液和试剂。
For isolation of viral RNA from cell-free body fluids
· Rapid isolation of high-quality, ready-to-use RNA
· No organic extraction or alcohol precipitation
· Consistent, high yields
· Complete removal of contaminants and inhibitors
The QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids with fast spin-column or vacuum procedures. Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. Purification can be fully
The QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids, offering fast spin-column or vacuum procedures, or automation on QIAcube. Sample types include:
Plasma and serum
CSF
Urine
Other cell-free body fluids
Cell-culture supernatants
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文献和实验application. 3. RNA Storage Store the purified RNA on ice for immediate use. For long–term storage, keep the purified RNA at –80°C. Perform DNase I treatment after purification (refer to the PureLink™ RNA Mini Kit manual) to assure highly
of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately and ready to use in any downstream application. The kit is also available in spin-column format for micro- and mini-sized samples. Principle and procedure
A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
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