RN001 RNA 快速提取试剂盒产品图
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RN001 RNA 快速提取试剂盒

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  • ¥980
  • 奕杉
  • RN001
  • 苏州
  • 2026年03月09日
    48h发货
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    • 详细信息
    • 用户评价
    • 文献和实验
    • 技术资料
    • 库存

      库存充足

    • 英文名

      RNA-Quick Purification Kit

    • 供应商

      奕杉

    • 保存条件

      Elution Buffer需要分装成小份室温保存,其余试剂和 RNA 柱室温保存 (使用中谨防试剂被污染)。(附赠的DNA 酶收到后需置于-20℃保存。)

    • 规格

    产品简介

    本试剂盒可以从少量的生物样品中快速提取高质量的总 RNA,  具有操作  简单快速,性能可靠,不使用酚、氯仿等有毒物质的优点。本产品适用于  细胞、特定类型的动物组织的 RNA  提取,提取得到的总 RNA  可用于  RT-PCRRT-qPCRNorthern blottingcDNA文库构建等多种实验。 本试剂盒提供的裂解液(Lysis  Buffer)中含有强变性剂,能够迅速裂解 样品并失活 RNA 酶,确保操作过程中RNA的完整性。本试剂盒大致的操  作过程如下:首先用裂解液裂解样品;裂解好的样品加入乙醇混匀即可上柱,离心去掉液体将RNA 结合在柱子上;杂质在清洗过程中被有效去除; 洗脱 RNA 并用于下游实验。

    产品内容

    组分

    RN001(100次)

    Lysis Buffer

    60 ml

    Wash Buffer*

    12 ml

    Elution Buffer

    25 ml

    RNA纯化柱(带收集管)

    100套

    *第一次使用前,须向Wash Buffer中加入48 ml 无水乙醇。

    保存条件

    Elution Buffer需要分装成小份室温保存,其余试剂和 RNA 柱室温保存

    (使用中谨防试剂被污染)。(附赠的DNA 酶收到后需置于-20℃保存。)

    关于试剂盒的适用性

    本产品仅用于科研,请勿用于医药、临床治疗、化妆品及食品等用途。

    用于细胞样品时建议用6孔板或35 mm   培养皿培养细胞,培养至合适的密度进行 RNA 提取(24孔板培养的细胞培养至90%以上的细胞密度也可使用)。不建议用100 mm  培养皿培养的细胞直接进行 RNA  提取,否则可能导致细胞裂解不充分或因核酸 过量导致离心柱堵塞或导致基因组残留。

    用于组织样品时建议用于内脏组织、肿瘤组织等,不适用于皮肤等坚韧的组织。

    该试剂盒可提取5~300万常规细胞或1~100 mg 组织的RNA  ,对 于T 细胞/B 细胞 等体积很小、RNA 含量很低的细胞,建议增加细胞数到至少100万以上。产品细节图片1

     

    产品细节图片2

    奕杉生物(ESScience)自2018年推出8分钟RNA快速提取试剂盒(货号:RN001)以来,就以其出色的品质和优质的价格在市场上广受好评,该产品发表的SCI文献已超百篇,总影响因子近千分,其中最高引用文献影响因子达38分(Cancer Cell),RNA快速提取试剂盒无疑是您分子实验的好帮手。

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