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- 英文名：
Rac1 G-LISA GTPase Activation Assay Kit (Colorimetric Based) 96 assays
- 库存：
1
- 规格：
96T
Rac1 G-LISA GTPase Activation Assay Kit (Colorimetric Based) 96 assays
The proprietary Rac1 G-LISA™ Activation Assay that is faster, easier and more precise than traditional pull-down methods to measure Rac1 Activation.
- Specific for Rac1
- Colorimetric Based
- Fast Results
- Extremely Sensitive
General Information
This Rac1 G-LISA™ is a colorimetric based assay and shares the many advantages that all G-LISA™ activation assay have. This assay is specific for Rac1.
The Rac1 G-LISA™ kit contains a Rac-GTP-binding protein linked to the wells of a 96 well plate. Active GTP-bound Rac1 is captured by this protein while inactive GDP-bound Rac1 is removed following washes. The active Rac1 bound to the wells is detected with a Rac1 specific antibody. The degree of Rac1 activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.
Kit Contents - Enough reagents for 96 assays (Stripwell)
96 individual Rac1-GTP binding wells
Anti-Rac1 antibody (Cat. # ARC03)
Secondary Antibody - HRP
Rac1 control protein
Cell Lysis Buffer
Wash buffer
Antigen Presenting Buffer
Antibody Dilution Buffer
HRP Detection and Stop Reagents
Precision Red™ Advanced Protein Assay (Cat. # ADV02)
Protease Inhibitor Cocktail (Cat. # PIC02)
Figure 1.The simple procedure of the Rac1 G-LISA™ assay.
Figure 2. Rac1 activation by EGF measured by G-LISA™. Swiss 3T3 cells were serum starved (SS) for 48 h and treated with EGF (10 ng/ml for 2 min). 12.5 μg and 25 μg of cell lysates were subjected to the G-LISA™ assay. Absorbance was read at 490 nm.
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文献和实验因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
Assessment of Rho GTPase Signaling During Neurite Outgrowth
Rho GTPases are key regulators of the cytoskeleton during the process of neurite outgrowth. Based on overexpression of dominant-positive and negative Rho GTPase constructs, the classic view is that Rac1 and Cdc42 are important for neurite
cells (hESC) are difficult to adapt to 96?well plate assays, such as the MTT assay, because they survive best when plated as colonies, which are not easily counted and plated accurately. Two methods were developed to address this problem
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