EasySep™
简单快捷的免疫磁珠细胞分选EasySep™是一种强大的免疫磁珠细胞分选平台，它结合了单克隆抗体的特异性和无柱磁珠分选平台的简便性，能够快速、简单地分离出高纯度的细胞群，且可立即用于下游应用。
通过使用对表面抗原具有特异性的抗体复合物，经标记的细胞可被去除（负选），也可被分选（正选）。抗体复合物可将目标细胞与磁珠相连接。当样本被置于EasySep™磁极中，经标记的细胞在磁场的 作用下将被吸附于试管壁上。未经标记的细胞则可被轻松倾倒或吸取到一个新的试管中，而被磁珠标记的细胞则保留在试管中。EasySep™用于人细胞
短至8分钟的免疫磁珠细胞分选 EasySep™试剂盒可用于从各种样本来源中分选人细胞，包括：外周血单个核细胞（PBMCs）、全血、白细胞单采术（Leukapheresis Pack）样本、骨髓和脐带血。目标细胞被抗体复合物和磁珠标记，以进行负选或正选。
使用我们新一代的EasySep™细胞分选试剂盒，短至8分钟，即可分选高纯度、未经标记的、功能性的人免疫细胞。操作流程
实验数据
图1. EasySep™人CD4+ T细胞分选试剂盒（产品号 #17952）
起始样本为人外周血单个核细胞，分选所得细胞中CD4+ T细胞（CD3+ CD4+）的含量通常可达94.8±2.3%（平均值±标准误差；以具活性的单线态细胞设门）。在以上示例中，起始样本和分选后的细胞的纯度分别为39.4%和98.2%。The EasySep™ Human CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
This product can be used in place of the EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052) for even faster cell isolations.
Advantages:• Fast, easy-to-use and column-free
• Up to 97% purity with high recovery
• Untouched, viable cells
Components:- EasySep™ Human CD4+ T Cell Isolation Kit (Catalog #17952)
- EasySep™ Human CD4+ T Cell Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Human CD4+ T Cell Isolation Kit (Catalog #17952RF)
- EasySep™ Human CD4+ T Cell Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyPlate™ EasySep™ Magnet (Catalog 18102)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Cell Type:T细胞; CD4+T细胞
Species:人
Sample Source:白细胞去除术; PBMC
