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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
American Diagnostica Inc
- 检测范围:
0 - 0.8 U/mL
- 检测方法:
发色底物法
- 应用:
枸橼酸钠抗凝血浆
- 样本:
25 μL
- 规格:
100 tests
ACTICHROME® Heparin (anti-FXa), activity assay
肝素抗Xa活性发色底物法试剂盒
INTENDED USE
ACTICHROME® Heparin (anti-FXa) is a chromogenic assay intended for the quantitative determination of unfractionated and low molecular weight heparins in human plasma by measurement of factor Xa inhibition.
PRINCIPLE
The inhibitory effect of antithrombin III (AT-III) on thrombin, factor Xa and other coagulation serine proteases in plasma is increased several thousandfold by heparin. This inhibition accounts for the anticoagulant effect of heparin. The quantitative determination of plasma heparin levels by the
measurement of their anti-Xa activity is a necessary tool for monitoring treatment efficacy.
Low molecular weight heparin (LMWH) preparations appear to catalyze the reaction between factor Xa and AT-III more readily than the reaction between thrombin (FIIa) and AT-III. Unfractionated heparin (UFH) catalyzes both reactions equally. The factor Xa inhibition test is the most useful assay
covering the widest variety of heparin preparations. In the assay, the rate of factor Xa inhibition is directly proportional to the heparin concentration since both factor Xa and AT-III are in excess. The residual factor Xa activity is inversely proportional to the heparin concentration.1,2
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文献和实验Are Low Molecular Weight Heparins the Same
them non-interchangeable. The anticoagulant potency of heparin is measured as USP U/mg. This method is only applicable to LMWHs at high doses, when their anticoagulant properties are apparent. Anti-Xa activity has not enabled standardization of the biologic
Glycosaminoglycan Binding Assays
-I activity of RANTES. Removal of cell-surface heparin sulfate, but not chon-droitin sulfate from a human T-cell line, rendered the cells resistant to the antiviral effects of RANTES (4 ). These authors demonstrate the importance of understanding
) (4) , to the final purified protein. In addition, protocolsto assay Wnt activity are described. Two key observations have made the purification of Wntspossible: 1) inclusion of detergent to facilitate solubility of thehighly hydrophobic Wnt protein
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