beta-Actin β-肌动蛋白/β-Actin单克隆抗体(内参抗体)

beta-Actin β-肌动蛋白/β-Actin单克隆抗体

(内参抗体)
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  • ¥580 - 1600
  • LMAI Bio
  • LM-33036M
  • 进口/国产
  • 2025年12月08日
  • WB=1:5000-20000 ELISA=1:5000-10000 (石蜡切片需做抗原修复)
  • Rabbit
  • Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, Fish, Guinea Pig, Hamster, Cat,
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    • 详细信息
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-33036M

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      beta-Actin

    • 抗体名

      β-肌动蛋白/β-Actin单克隆抗体(内参抗体)

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, Fish, Guinea Pig, Hamster, Cat,

    • 免疫原

      KLH conjugated synthetic peptide derived from human beta-Actin:

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      WB=1:5000-20000 ELISA=1:5000-10000 (石蜡切片需做抗原修复)

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      50ul  100ul  200ul

    beta-Actin β-肌动蛋白/β-Actin单克隆抗体(内参抗体)
    英文名称 beta-Actin
    中文名称 β-肌动蛋白/β-Actin单克隆抗体(内参抗体)
    别    名 Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.  
    规格价格 50ul/580元 购买    100ul/920元 购买    200ul/1600元 购买    大包装/询价
    说 明 书 50ul  100ul  200ul
    产品类型 内参抗体 
    研究领域 肿瘤  细胞生物  信号转导  细胞骨架  
    抗体来源 Mouse
    克隆类型 Monoclonal
    克 隆 号 1A2
    交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, Fish, Guinea Pig, Hamster, Cat, 
    产品应用 WB=1:5000-20000 ELISA=1:5000-10000 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 42kDa
    细胞定位 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human beta-Actin: 
    亚    型 IgG
    纯化方法 affinity purified by Protein G
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Loading Control
    This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].

    Function:
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

    Subunit:
    Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2.

    Subcellular Location:
    Cytoplasm. cytoskeleton.

    Tissue Specificity:
    Ubiquitously expressed in all eukaryotic cells.

    Post-translational modifications:
    ISGylated.
    Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

    DISEASE:
    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.

    Similarity:
    Belongs to the actin family.

    SWISS:
    P60709

    Gene ID:
    60

    Database links:

    Entrez Gene: 396526 Chicken

    Entrez Gene: 60 Human

    Entrez Gene: 11461 Mouse

    Entrez Gene: 100009272 Rabbit

    Entrez Gene: 81822 Rat

    Omim: 102630 Human

    SwissProt: P60706 Chicken

    SwissProt: P60712 Cow

    SwissProt: P60708 Horse

    SwissProt: P60709 Human

    SwissProt: P60710 Mouse

    SwissProt: P29751 Rabbit

    SwissProt: P60711 Rat

    SwissProt: P60713 Sheep

    Unigene: 520640 Human

    Unigene: 708120 Human

    Unigene: 727576 Human

    Unigene: 328431 Mouse

    Unigene: 391967 Mouse

    Unigene: 94978 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 beta-Actin β-肌动蛋白/β-Actin单克隆抗体
    Sample: 
    293T Cell (Human) Lysate at 40 ug
    NIH/3T3 Cell (Mouse) Lysate at 40 ug
    Muscle (Mouse) Lysate at 40 ug
    Primary: Anti-β-Actin (bsm-33036M) at 1/5000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 42 kD

    beta-Actin β-肌动蛋白/β-Actin单克隆抗体
    Sample: 
    A549(Human) Cell Lysate at 30 ug
    Primary: Anti-beta-Actin (bsm-33036M) at 1/500 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 42 kD

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