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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-33036M
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
beta-Actin
- 抗体名:
β-肌动蛋白/β-Actin单克隆抗体(内参抗体)
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, Fish, Guinea Pig, Hamster, Cat,
- 免疫原:
KLH conjugated synthetic peptide derived from human beta-Actin:
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:5000-20000 ELISA=1:5000-10000 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
50ul 100ul 200ul
| 英文名称 | beta-Actin |
| 中文名称 | β-肌动蛋白/β-Actin单克隆抗体(内参抗体) |
| 别 名 | Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN. |
| 规格价格 | 50ul/580元 购买 100ul/920元 购买 200ul/1600元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 产品类型 | 内参抗体 |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞骨架 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 1A2 |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, Fish, Guinea Pig, Hamster, Cat, |
| 产品应用 | WB=1:5000-20000 ELISA=1:5000-10000 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 42kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human beta-Actin: |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Loading Control This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]. Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Subunit: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Subcellular Location: Cytoplasm. cytoskeleton. Tissue Specificity: Ubiquitously expressed in all eukaryotic cells. Post-translational modifications: ISGylated. Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced. DISEASE: Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed. Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent. Similarity: Belongs to the actin family. SWISS: P60709 Gene ID: 60 Database links: Entrez Gene: 396526 Chicken Entrez Gene: 60 Human Entrez Gene: 11461 Mouse Entrez Gene: 100009272 Rabbit Entrez Gene: 81822 Rat Omim: 102630 Human SwissProt: P60706 Chicken SwissProt: P60712 Cow SwissProt: P60708 Horse SwissProt: P60709 Human SwissProt: P60710 Mouse SwissProt: P29751 Rabbit SwissProt: P60711 Rat SwissProt: P60713 Sheep Unigene: 520640 Human Unigene: 708120 Human Unigene: 727576 Human Unigene: 328431 Mouse Unigene: 391967 Mouse Unigene: 94978 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: 293T Cell (Human) Lysate at 40 ug NIH/3T3 Cell (Mouse) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Primary: Anti-β-Actin (bsm-33036M) at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD ![]() Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-beta-Actin (bsm-33036M) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD |
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文献和实验小鼠β-肌动蛋白(β-actin)酶联免疫分析(ELISA)
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