- ¥1380 - 2200
- LMAI Bio
- LM-9597R
- 进口/国产
- 2026年01月09日
- WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-9597R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Cdc25C
- 抗体名：
细胞分裂周期蛋白25C抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Pig,
- 免疫原：
KLH conjugated synthetic peptide derived from human Cdc25C:261-360/473
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul 200ul
Cdc25C细胞分裂周期蛋白25C抗体
| 英文名称 | Cdc25C |
| 中文名称 | 细胞分裂周期蛋白25C抗体 |
| 别 名 | CDC 25; Cdc 25C; CDC25; Cell division cycle 25 homolog C; Cell division cycle 25C; Cell division cycle 25C protein; Dual specificity phosphatase Cdc25C; M phase inducer phosphatase 3; Mitosis inducer CDC25; MPIP3; Phosphotyrosine phosphatase; MPIP3_HUMAN. |
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Specific References (2) | bs-9597R has been referenced in 2 publications. [IF=3.73] Ghate, Nikhil Baban, et al. "An Antioxidant Extract of Tropical Lichen, Parmotrema reticulatum, Induces Cell Cycle Arrest and Apoptosis in Breast Carcinoma Cell Line MCF-7." PLOS ONE 8.12 (2013): e82293. WB ; Human. PubMed:24358166 [IF=0.00] Ghate, N. B., et al. "Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio." Cell Death Discovery 2 (2016). WB ; Human. PubMed:27551490 |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 信号转导 细胞周期蛋白 激酶和磷酸酶 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, |
| 产品应用 | WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 53kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cdc25C:261-360/473 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008] Function: Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity. Subunit: Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity. Interacts with MAPK14 and 14-3-3 proteins. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity. Similarity: Belongs to the MPI phosphatase family. Contains 1 rhodanese domain. SWISS: P30307 Gene ID: 995 Database links: Entrez Gene: 995 Human Omim: 157680 Human SwissProt: P30307 Human Unigene: 656 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CDC25家族的组成哺乳动物中CDC25家族一共包括3种同源异构体（CDC25A、CDC25B、CDC25C），约有50%的序列同源，是一组在细胞周期调控中发挥巨大作用的苏/酪氨酸双功能酶 。不同的CDC25家族蛋白在细胞周期中的作用时相亦有差异，CDC25A和CDC25C分别在S期和M期发挥主要作用。 |
| 产品图片 |  Sample: Intestine (Mouse) Lysate at 40 ug Testis (Mouse) Lysate at 40 ug Primary: Anti-Cdc25C (bs-9597R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 53 kD Observed band size: 53 kD  Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cdc25C) Polyclonal Antibody, Unconjugated (bs-9597R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cdc25c Polyclonal Antibody, Unconjugated(bs-9597R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cdc25C Polyclonal Antibody, Unconjugated(bs-9597R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: mouse splenocytes(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green). |
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文献和实验相关实验
引言 在哺乳动物的早期发育过程中，胚胎干细胞 (ESC) 经历细胞分化并产生所有胚胎生殖层，这些层最终在成年生物体中分化为多种细胞类型。 ESC 的特点是具有难以置信的自我更新和增殖能力，由于间隙期（G1 和 G2）截短，因此其分裂周期较短。 在细胞分化过程中，会发生标志性的细胞事件： 细胞改变形态，生长至约为其原有大小的十倍，并改变细胞核与细胞质的比率，同时细胞变得扁平和拉长； 命运特异性基因表达程序被激活，并发生全局染色质修饰。 重要的是，细胞周期减慢。 分化的体细胞具有较长的G1期和G2
抗体的结构决定了其对应的功能，同一抗体的V区和C区的氨基酸组成和顺序不同，决定了其功能上的差异。V区和C区的组成和结构，决定了抗体的生物学功能。 图1：抗体的主要功能（图片来源：Abbas et al: Cellular and Molecular Immunology, 7e.) 1、特异性识别功能 抗体可以通过其结构上的可变区域与抗原结合，同时，具有极高的特异性。这种特异性识别功能可以使抗体识别和结合到感染的病原体，肿瘤细胞等不同种类的分子，帮助免疫系统识别并定位到这些分子，进而启动
着两者都可，那就都能做。 3、石蜡切片的优点是可以保持组织细胞的形态结构，且容易存放在室温，而冰冻切片比较麻烦，一定要存在 -80ºC 的低温冰箱中，尤其是用来做原位杂交的切片，为了防止 RNA 降解，保存一贯很重要。由于石蜡切片可以切到 4 μm 左右，所以原位杂交探针容易渗透到组织中去，容易成功，而且得到的颜色/形态都较冰冻切片好。 二、一抗的选择要点和技巧是什么？ 1、单克隆和多克隆抗体的选择。由一种克隆产生的特异性抗体叫做单克隆抗体。单克隆抗体能目标明确地与单一的特异抗原决定簇结合，就像导弹精确
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Cdc25C细胞分裂周期蛋白25C抗体
¥1380 - 2200
