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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-9594R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
PINX-1
- 抗体名:
端粒酶抑制因子PinX-1抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Pig, Cow, Horse,
- 免疫原:
KLH conjugated synthetic peptide derived from human PINX-1:65-160/328
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | PINX-1 |
| 中文名称 | 端粒酶抑制因子PinX-1抗体 |
| 别 名 | 67-11-3 protein; FLJ20565; Hepatocellular carcinoma-related putative tumor suppressor; Liver-related putative tumor suppressor; LPTL; LPTS; MGC8850; PIN2 interacting protein 1; Pin2-interacting protein X1; PIN2/TERF1-interacting telomerase inhibitor 1; PINX1; PINX1_HUMAN; Protein 67-11-3; TRF1-interacting protein 1. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, Horse, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 37kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PINX-1:65-160/328 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: PINX1 is a ubiquitously expressed protein that localizes to nucleoli and telomere speckles. PINX1 contains one G-patch domain and one telomeric inhibiting domain (TID) at its C-terminus. PINX1 interacts with the telomere protein TRF1 and the telomerase reverse transcriptase TERT. The TID domain of PINX1 specifically interacts with TERT and functions to inhibit its activity, thus participating in the regulation of telomerase activity. Overexpression of PINX1 leads to shortened telomeres, further supporting an inhibitory role of PINX1 on telomerase activity. The depletion of PINX1 significantly increases telomerase activity and may lead to tumorigenicity of cancer cells. This suggests that PINX1 acts as a tumor suppressor and can inhibit cell proliferation. In addition, PINX1 is involved in nucleolar RNA maturation. Function: Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor. Subunit: Interacts with MCRS1, TERT, TERF1, NCL/nucleolin, and the telomerase RNA. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous; expressed at low levels. Similarity: Belongs to the PINX1 family. Contains 1 G-patch domain. SWISS: Q96BK5 Gene ID: 54984 Database links: Entrez Gene: 54984 Human Entrez Gene: 72400 Mouse Entrez Gene: 305963 Rat Omim: 606505 Human SwissProt: Q548A5 Human SwissProt: Q6QWG9 Human SwissProt: Q96BK5 Human SwissProt: Q9CZX5 Mouse SwissProt: A4L691 Rat Unigene: 490991 Human Unigene: 583894 Human Unigene: 379214 Mouse Unigene: 15365 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PINX-1 Polyclonal Antibody, Unconjugated(bs-9594R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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文献和实验它可能作为肝转移的一个诊断标志。4. 微卫星DNA 在人类基因组中有许多CA、GT等散在的2-4个碱基对的单纯反复排列,称为微卫星。有关它的功能还不明了。据报道,在家族性非息肉性大肠癌中以及散在性大肠癌中有高比率的微卫星反复排列数量的异常。5. 端粒酶 位于染色体末端的端粒在人体中是由T、T、A、G、G、G6个碱基构成一个单位,在生殖细胞中以长约20kb,体细胞中以6-10kb的长度进行反复排列,通常认为端粒与染色体的稳定性有关,其长度随着细胞的反复分裂而缩短,缩短到一定程度细胞再分裂,也就是说
的DNA 区域。 Marker (DNA 标记):已知大小的DNA 片段,用来计算琼脂糖凝胶电泳条带。 Marker (genetic ) (遗传标记):在试验中任何感兴趣的等位基因。 Maternal inheritance (母性遗传):指只有一个亲本提供的遗传标记在子代更容易存活。 Mb(兆碱基):106 bp DNA 的缩写。 Meiosis (减数分裂):由两次连续的分裂(减数分裂I期,减数分裂II期),使最初的4n 染色 体减少为4 个1n 染色体的产物细胞。产物
质质从内质网沿着高尔基体向质膜转运。 Antibody (抗体):由B 淋巴细胞产生的蛋白质(免疫球蛋白质),它能识别特殊的外源“抗原”,从而引起免疫应答。 Anticoding strand (反编码链):DNA 双链中作为膜板指导与之互补的RNA 合成的链。 Antigen (抗原):进入基体后能引起抗体(免疫球蛋白质)合成的分子。 Antiparallel (反式平行):DNA双螺旋以相反的方向组织,因此一条链的5‘端与另一条链的3‘端相连。
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