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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-9432R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
GERP
- 抗体名:
环指蛋白27抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Chicken, Dog, Horse, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human GERP/TRIM8/RNF27:61-160/551
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | GERP |
| 中文名称 | 环指蛋白27抗体 |
| 别 名 | RNF27; Lioblastoma expressed ring finger protein; Ring finger protein 27; RNF27; RP11-47A8.4; TRIM8; Tripartite motif containing 8; Tripartite motif protein TRIM8; TRIM8_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Horse, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 61kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GERP/TRIM8/RNF27:61-160/551 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM8 (tripartite motif containing 8), also known as GERP (glioblastoma-expressed RING finger protein) or RNF27 (RING finger protein 27), is a 551 amino acid protein that is thought to function as an E3 ubiquitin-protein ligase that promotes SOCS-1 proteasomal degradation. As a widely expressed homodimer, TRIM8 localizes to nuclear bodies and contains two B box-type zinc fingers and one RING-type zinc finger. TRIM8 is expressed in lung, heart, brain and skeletal muscle, with low levels detected in intestine, placenta, leukocytes and liver. The gene encoding TRIM8 maps to human chromosome 10q24.32. Function: Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1. Subunit: Homodimer. Interacts with SOCS1 (via) SH2 domain and SOCS box. Tissue Specificity: Widely expressed. Similarity: Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 RING-type zinc finger. SWISS: Q9BZR9 Gene ID: 81603 Database links: Entrez Gene: 81603 Human Entrez Gene: 93679 Mouse Entrez Gene: 688785 Rat Omim: 606125 Human SwissProt: Q9BZR9 Human SwissProt: Q99PJ2 Mouse Unigene: 336810 Human Unigene: 392177 Mouse Unigene: 481672 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 葛博:bs-9432R 细胞定位:细胞质 细胞核 2017.11.08日张凤英修改 |
| 产品图片 | ![]() Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti- GERP (bs-9432R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61kD Observed band size: 61kD ![]() Sample: HepG2 Cell (Human) Lysate at 30 ug Hela Cell(Human)Lysate at 30 ug Primary: Anti- GERP (bs-9432R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61kD Observed band size: 61kD |
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文献和实验Nature 综述导读:FDA 批准第 100 个抗体药物!抗体药物的研究方向在哪里?
人源化单克隆抗体成功转化到临床。在全球范围内,至少有 570 种治疗性单克隆抗体正在临床试验中,其中有 79 种治疗性单克隆抗体已获得美国 FDA 批准。图四 Journal of Biomedical Science (2020) 27:1自 1986 年的第一个获批单抗药物以来,FDA 直到 8 年后的 1994 年才批准第二种治疗性单克隆抗体产品。2013 年之前,每年也只有寥寥几个抗体获批上市,但是近十年以来,抗体的获批已慢慢开始变得稀松平常,平均每年约有 10 项批准。FDA 于 2015 年
抗原修复方法 Antigen Retrieval Methods
CTS013)和pH值为9.5的碱性抗原修复液(货号CTS013)中孵育后,p27的检出率显著提高,但在pH值为5.0的酸性抗原修复液(货号CTS014)中则无此效果。p27的检测采用抗人/小鼠/大鼠p27单克隆抗体(货号MAB22561;棕色)。
-qPCR 也检测到很多位点的 H3K27me3 水平有明显下调。 但用常规的 ChIP-seq 分析方法比较,并不能检测到 H3K27me3 水平的下降。造成这个结果的原因在于在标准的 ChIP-seq 实验过程中,文库构建需要的 PCR 扩增以及常规数据分析时对整体数据量的校正掩盖了样本间数据量的差异。 针对这一问题,Active Motif 凭借多年的 ChIP-seq 服务经验成功研发出「Spike-In」校正策略。标准的 ChIP 反应是使用样本染色质和抗体建立
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