- ¥780 - 2200
- LMAI Bio
- LM-2593R
- 进口/国产
- 2025年12月12日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Dog, Rabbit,
9 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-2593R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Caspase 3 precursor
- 抗体名：
半胱天冬酶-3酶原抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog, Rabbit,
- 免疫原：
KLH conjugated synthetic peptide derived from human Caspase 3 precursor:11-120/277
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
Caspase 3 precursor半胱天冬酶-3酶原抗体
| 英文名称 | Caspase 3 precursor |
| 中文名称 | 半胱天冬酶-3酶原抗体 |
| 别 名 | Caspase 3 precursor; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1. |
 |
Specific References (3) | bs-2593R has been referenced in 3 publications.
[IF=1.06] Zhang, Xiaolin, and Hao Yu. "Matrine inhibits diethylnitrosamine-induced HCC proliferation in rats through inducing apoptosis via p53, Bax-dependent caspase-3 activation pathway and down-regulating MLCK overexpression (Supplement 2016)." Iranian Journal of Pharmaceutical Research (2016). WB ; Rat.
PubMed:27642320
[IF=3.85] Luo, Xiaoming, et al. "Pharmacokinetics and antitumor efficacy of micelles assembled from multiarmed amphiphilic copolymers with drug conjugates in comparison with drug-encapsulated micelles." European Journal of Pharmaceutics and Biopharmaceutics (2015). IHC-P ; Mouse.
PubMed:26523356
[IF=5.74] Duan, Xiaoxu, et al. "Antioxidant tert-butylhydroquinone ameliorates arsenic-induced intracellular damages and apoptosis through induction of Nrf2-dependent antioxidant responses as well as stabilization of anti-apoptotic factor Bcl− 2 in human keratinocytes." Free Radical Biology and Medicine(2016). WB ; Human.
PubMed:26878773
|
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 28kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Caspase 3 precursor:11-120/277 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa. Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Subcellular Location: Cytoplasm. Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Post-translational modifications: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. Similarity: Belongs to the peptidase C14A family. SWISS: P55213 Gene ID: 836 Database links: Entrez Gene: 836 Human Entrez Gene: 12367 Mouse Entrez Gene: 397244 Pig Entrez Gene: 100008840 Rabbit Entrez Gene: 25402 Rat Omim: 600636 Human SwissProt: P42574 Human SwissProt: P70677 Mouse SwissProt: Q95ND5 Pig SwissProt: Q8MJC3 Rabbit SwissProt: P55213 Rat Unigene: 141125 Human Unigene: 34405 Mouse Unigene: 10562 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
Sample:
Spleen (Mouse) Lysate at 40 ug Primary: Anti-Caspase 3 precursor (bs-2593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD 
Sample:
Lung (Mouse) Lysate at 40 ug Primary: Anti-Caspase 3 precursor (bs-2593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD 
Sample:
Liver (Mouse) Lysate at 40 ug Primary: Anti-Caspase 3 precursor (bs-2593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD 
Sample:
Lane1: Brain (Mouse) Lysate at 30 ug Lane2: Liver (Mouse) Lysate at 30 ug Primary: Anti-pro-caspase-3 (bs-2593R) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1:3000 dilution; Predicted band size : 28kD Observed band size : 30kD 
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 3 precursor) Polyclonal Antibody, Unconjugated (bs-2593R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-pro-caspase-3 Polyclonal Antibody, Unconjugated(bs-2593R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-Caspase 3 precursor antibody (bs-2593R), Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1μg /test. 
Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-Caspase 3 precursor antibody (bs-2593R), Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
Caspase-3（CPP-32，Apoptain，Yama，SCA-1），半胱天冬酶家族成员之一，是凋亡的关键执行者，对许多关键的蛋白起着部分或全部的裂解作用，如核酶多聚（ADP-ribose）聚合酶（PARP）[1] 及众多细胞骨架相关的蛋白、激酶以及 Bcl-2 家族中凋亡相关的蛋白等[2] 。Caspase-3 在肺、脾脏、心脏、肝脏、肾脏等中广泛表达，在大脑和骨骼肌中表达量稍低，在睾丸中表达量最低。同时在许多细胞系中都有发现 caspase-3 的表达，其中免疫相关的细胞中表达量最高
Caspase8 及其介导的级联反应 Caspase中的“C”代表半胱氨酸(cysteine)，“asp"指天冬氨酸(aspasticacid)。因而caspase可以称为胱天蛋白酶，或天冬氨酸特异性半胱氨酸蛋白酶，因为其专一性地在天冬氨酸之后切断与另一氨基酸残基的连接使底物解离。目前已发现的caspase至少有12种，按其发现的先后，在caspase后面冠以阿拉伯数字表示。 半胱天冬蛋白酶家族成员及其功能 Caspase家族一般具有如下特征：①C端同源区存在牛
式途径是通过TNF受体家族（如CD95）的受体与配体结合开始的。这些配体有癌症坏死因子（TNF），和其他细胞因子，后者可以由如T淋巴细胞分泌。 在FADD（Fas偶联死亡区域蛋白Fas-associated death domain protein）的协助下，受体不断在细胞质中收集半胱天冬酶原8（Procaspase 8）。后者通过高密度自催化激活自身。活化的半胱天冬酶8（Caspase 8）将会引发所谓的Caspase级联反应。因此在艾滋病人体内，很多未受感染的白血球也会凋亡：HIV
技术资料暂无技术资料 索取技术资料
Caspase 3 precursor半胱天冬酶-3酶原抗体
¥780 - 2200
