Anti-Human GM-CSF

Anti-Human GM-CSF

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  • ¥3988
  • Biogems
  • 2025年07月09日
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    • 详细信息
    • 技术资料
    • 克隆性

      多克隆

    • 供应商

      安诺伦(北京)生物科技有限公司

    • 靶点

      Recombinant Human GM-CSF (BioGems Catalog #300-03)

    • 规格

      50μg

    来源:Polyclonal Rabbit

    存储溶液:purified

    官网链接:http://www.bio-gems.com/recombinant-proteins/anti-cytokine-chemokine-antibodies/anti-human-gm-csf.html

    商品关键词:Anti-Human GM-CSF,500-P33,50μg,

    简单描述:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGM-CSF. Anti-Human GM-CSF specific antibody was purified by affinity chromatography employing immobilized hGM-CSF matrix.

    Immunohistochemistery: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.25 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

    Sandwich ELISA: To detect hGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human GM-CSF (60-167BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGM-CSF.

    Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGM-CSF (1.0 ng/ml), a concentration of 0.13-0.20 µg/ml of this antibody is required.

    官网链接: www.annoron.com
    办公电话: 010-56256916
    官方售后: 400-965-8633
    企业QQ: 3198592576、3212874516

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