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Ribonuclease Inhibitor核糖核酸酶抑制因

子1抗体
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  • ¥1380 - 2200
  • LMAI Bio
  • LM-7518R
  • 进口/国产
  • 2025年11月13日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
  • Rabbit
  • Human, Mouse, Rat, Pig, Cow,
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-7518R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      Ribonuclease Inhibitor

    • 抗体名

      核糖核酸酶抑制因子1抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Pig, Cow,

    • 免疫原

      KLH conjugated synthetic peptide derived from hu Ribonuclease Inhibitor:101-200/461

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      100ul  200ul

    Ribonuclease Inhibitor核糖核酸酶抑制因子1抗体
    英文名称 Ribonuclease Inhibitor
    中文名称 核糖核酸酶抑制因子1抗体
    别    名 Placental ribonuclease inhibitor; RNH; RNH1; Placental RNase inhibitor; PRI; RAI; RI antibody Ribonuclease inhibitor; Ribonuclease/angiogenin inhibitor 1; Ribonuclease/angiogenin inhibitor; RINI_HUMAN; RNase inhibitor; RNH 1; Rnh1 ribonuclease/angiogenin inhibitor 1.  
    产品细节图片1 Specific References  (1)     |     bs-7518R has been referenced in 1 publications.
    [IF=1.60] Tang, Y., et al. "Overexpression of ribonuclease inhibitor defines good prognosis and suppresses proliferation and metastasis in human colorectal cancer cells via PI3K/AKT pathway." Clinical and Translational Oncology: 1-8.  IHC-P ;  Human.  
    PubMed:25257839
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 细胞生物  信号转导  表观遗传学  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Pig, Cow, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 50kDa
    细胞定位 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from hu Ribonuclease Inhibitor:101-200/461 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play role in redox homeostasis.

    Subunit:
    Forms high-affinity heterodimers with RNASE1, ANG and RNASE2.

    Subcellular Location:
    Cytoplasm.

    Post-translational modifications:
    The N-terminus is blocked.
    At least 30 of the 32 cysteine residues are in the reduced form.

    Similarity:
    Contains 15 LRR (leucine-rich) repeats.

    SWISS:
    P13489

    Gene ID:
    6050

    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 产品细节图片2
    Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (bs-7518R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
    产品细节图片3
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (bs-7518R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
    产品细节图片4
    Blank control(blue): Hela cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). 
    Primary Antibody:Rabbit Anti- Ribonuclease Inhibitor antibody(bs-1638R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; 
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); 
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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    图标文献和实验
    相关实验
    • The ribonuclease protection assay (RPA)

      The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA polymerases from the bacteriophages SP6, T

    • Ribonuclease Protection

      The ribonuclease protection assay (RPA) is a sensitive technique for the analysis of total cellular RNA. It involves generating a specific antisense riboprobe, hybridizing the probe to total RNA, removing unprotected RNA by RNases, and finally

    • Ribonuclease Inhibitors

      (Mg2+ , Mn2+ ), an RNA sample is most likely to be rapidly degraded by a contaminating ribonuclease (RNase). RNases are difficult to completely remove or inactivate during RNA isolation procedures, and they may be introduced into the sample

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