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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
1000⁺箱
- 保修期：
1月
- 现货状态：
现货
- 供应商：
上海睿安生物19121878899
- 规格：
80个/箱
Nunc货号150468现货EasYDish 150mm培养皿19121878899上海睿安生物
150mm培养皿，NunclonTM△，带盖，透气
Thermo Scientific™ Nunc™ EasYDish™ Dishes are the next generation of cell culture dishes that improve handling, stacking and transporting of cell cultures in your lab. The beveled grip ring is easy to hold and stays secure in your gloved hand. The dishes receive the standard Nunclon Delta surface treatment to maximize adhesion for majority of cell types. Available in four sizes: 35mm, 60mm, 100mm and 150mm.
描述
Beveled Grip Ring
- Ensures lid stays in position when dish is being handled
- Makes it easier to grasp and manage with a gloved hand
Proprietary Notch Design
- Maximizes ventilation while maintaining sterility
- Restricts the movement of the lid ensuring secure lid-to-dish fitting
- Increases the physical strength of the dish
Raised Outer Edge on Dish Lid
- Allows stabled stacking during transportation of cell cultures
Orientation Marks on Dish Bottom
- Simplifies locating of cells under the microscope
Writing Area
- Improves traceability of cell cultures
Re-Sealable Packaging
- Promotes cleanliness of opened packs
Specifications:
- Material: Polystyrene, USP VI
- Contaminant Free: RNase/DNase free, Human DNA free, non-cytotoxic, non-pyrogenic
- Sterility: Gamma Irradiation to SAL of 10-6
- Shelf Life: 5 years from manufacturing date
- Certificate of Quality in every case
- Surface Treatment: Nunclon Delta for adherent cell culture
-
Product Code Product Description (Chinese) Brand(r) Qty/PK Qty/CS 140640 Nunc插入式细胞培养皿，已灭菌，孔径大小，0.4um，建议工作容量，1.5ml Nunc 24 140660 Nunc插入式细胞培养皿，已灭菌，孔径大小，0.4um，建议工作容量，4.1ml Nunc 24 140663 Nunc插入式细胞培养皿，已灭菌，孔径大小，3um，建议工作容量，4.1ml Nunc 24 140668 Nunc插入式细胞培养皿，已灭菌，孔径大小，8um，建议工作容量，4.1ml Nunc 24 140652 Nunc插入式细胞培养皿，已灭菌，孔径大小，0.4um，建议工作容量，1.1ml Nunc 48 140654 Nunc插入式细胞培养皿，已灭菌，孔径大小，3um，建议工作容量，1.1ml Nunc 48 140620 Nunc插入式细胞培养皿，已灭菌，孔径大小，0.4um，建议工作容量，0.5ml Nunc 48 140627 Nunc插入式细胞培养皿，已灭菌，孔径大小，3um，建议工作容量，0.5ml Nunc 48 140629 Nunc插入式细胞培养皿，已灭菌，孔径大小，8um，建议工作容量，0.5ml Nunc 48 140656 Nunc插入式细胞培养皿，已灭菌，孔径大小，8um，建议工作容量，1.1ml Nunc 48 140644 Nunc插入式细胞培养皿，已灭菌，孔径大小，8um，建议工作容量，1.5ml Nunc 24 140642 Nunc插入式细胞培养皿，已灭菌，孔径大小，3um，建议工作容量，1.5ml Nunc 24 150265 CE认证的IVF ICSI培养皿，规格9×51mm Nunc 1 120 150270 CE认证的IVF细胞培养皿，规格60×15mm，已灭菌，聚苯乙烯 Nunc 10 400 150260 CE认证的IVF 中央孔板，规格16×55mm Nunc 1 120 137860 CE认证的IVF 11ml离心管, PS材质, RCF3000 Nunc 5 300 150268 14ml圆底试管，仅用于研究 Nunc 10 500 150255 CE认证的IVF细胞培养皿，规格35×10mm，已灭菌，聚苯乙烯 Nunc 10 500 150360 CE认证的IVF细胞培养皿，规格90×17mm，未经处理，透气式 Nunc 10 150 150460 35mm培养皿，NunclonTM△，带盖，透气 Nunc 10 500 150462 60mm培养皿，NunclonTM△，带盖，透气 Nunc 10 280 150464 100×15mm培养皿，NunclonTM△，带盖，透气 Nunc 10 150 150466 100×20mm培养皿，NunclonTM△，带盖，透气 Nunc 10 240 150468 150mm培养皿，NunclonTM△，带盖，透气 Nunc 10 80
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文献和实验Nunc货号150468现货EasYDish 150mm培养皿19121878899上海睿安生物
150mm培养皿，NunclonTM△，带盖，透气
Thermo Scientific™ Nunc™ EasYDish™ Dishes are the next generation of cell culture dishes that improve handling, stacking and transporting of cell cultures in your lab. The beveled grip ring is easy to hold and stays secure in your gloved hand. The dishes receive the standard Nunclon Delta surface treatment to maximize adhesion for majority of cell types. Available in four sizes: 35mm, 60mm, 100mm and 150mm.
描述
Beveled Grip Ring
- Ensures lid stays in position when dish is being handled
- Makes it easier to grasp and manage with a gloved hand
Proprietary Notch Design
- Maximizes ventilation while maintaining sterility
- Restricts the movement of the lid ensuring secure lid-to-dish fitting
- Increases the physical strength of the dish
Raised Outer Edge on Dish Lid
- Allows stabled stacking during transportation of cell cultures
Orientation Marks on Dish Bottom
- Simplifies locating of cells under the microscope
Writing Area
- Improves traceability of cell cultures
Re-Sealable Packaging
- Promotes cleanliness of opened packs
Specifications:
- Material: Polystyrene, USP VI
- Contaminant Free: RNase/DNase free, Human DNA free, non-cytotoxic, non-pyrogenic
- Sterility: Gamma Irradiation to SAL of 10-6
- Shelf Life: 5 years from manufacturing date
- Certificate of Quality in every case
- Surface Treatment: Nunclon Delta for adherent cell culture
-
Product Code Product Description (Chinese) Brand(r) Qty/PK Qty/CS 140640 Nunc插入式细胞培养皿，已灭菌，孔径大小，0.4um，建议工作容量，1.5ml Nunc 24 140660 Nunc插入式细胞培养皿，已灭菌，孔径大小，0.4um，建议工作容量，4.1ml Nunc 24 140663 Nunc插入式细胞培养皿，已灭菌，孔径大小，3um，建议工作容量，4.1ml Nunc 24 140668 Nunc插入式细胞培养皿，已灭菌，孔径大小，8um，建议工作容量，4.1ml Nunc 24 140652 Nunc插入式细胞培养皿，已灭菌，孔径大小，0.4um，建议工作容量，1.1ml Nunc 48 140654 Nunc插入式细胞培养皿，已灭菌，孔径大小，3um，建议工作容量，1.1ml Nunc 48 140620 Nunc插入式细胞培养皿，已灭菌，孔径大小，0.4um，建议工作容量，0.5ml Nunc 48 140627 Nunc插入式细胞培养皿，已灭菌，孔径大小，3um，建议工作容量，0.5ml Nunc 48 140629 Nunc插入式细胞培养皿，已灭菌，孔径大小，8um，建议工作容量，0.5ml Nunc 48 140656 Nunc插入式细胞培养皿，已灭菌，孔径大小，8um，建议工作容量，1.1ml Nunc 48 140644 Nunc插入式细胞培养皿，已灭菌，孔径大小，8um，建议工作容量，1.5ml Nunc 24 140642 Nunc插入式细胞培养皿，已灭菌，孔径大小，3um，建议工作容量，1.5ml Nunc 24 150265 CE认证的IVF ICSI培养皿，规格9×51mm Nunc 1 120 150270 CE认证的IVF细胞培养皿，规格60×15mm，已灭菌，聚苯乙烯 Nunc 10 400 150260 CE认证的IVF 中央孔板，规格16×55mm Nunc 1 120 137860 CE认证的IVF 11ml离心管, PS材质, RCF3000 Nunc 5 300 150268 14ml圆底试管，仅用于研究 Nunc 10 500 150255 CE认证的IVF细胞培养皿，规格35×10mm，已灭菌，聚苯乙烯 Nunc 10 500 150360 CE认证的IVF细胞培养皿，规格90×17mm，未经处理，透气式 Nunc 10 150 150460 35mm培养皿，NunclonTM△，带盖，透气 Nunc 10 500 150462 60mm培养皿，NunclonTM△，带盖，透气 Nunc 10 280 150464 100×15mm培养皿，NunclonTM△，带盖，透气 Nunc 10 150 150466 100×20mm培养皿，NunclonTM△，带盖，透气 Nunc 10 240 150468 150mm培养皿，NunclonTM△，带盖，透气 Nunc 10 80
冰上，用冰冷的 PBS 洗涤细胞。2. 吸出 PBS，然后加入冰冷的裂解缓冲液（每 107 细胞/100 mm 培养皿/150 cm2 培养瓶加入 1 ml；每 5×106 细胞/60 mm 培养皿/75 cm2 培养瓶加入 0.5 ml）。3. 用预冷的塑料细胞刮棒刮下贴壁细胞，然后轻轻地将细胞悬浮液转移至预冷的微量离心管中。4. 在 4 ℃ 下持续搅拌 30 分钟。5. 在 4 ℃ 预冷的离心机中以 16000 × g 的转速离心 20 分钟。6. 轻轻地从离心机中取出离心管，置于冰上。将上清
)；DMEM培养液（含4000 mg/L D-葡萄糖，4 mmol/L L-谷氨酰胺，添加3.7 g/L NaHCO3，20 mmol/L HEPES，肝素钠 100 mg/L，青霉素 100 U/ml，链霉素 100 ug/ml），pH值调至7.4，过滤除菌后分装保存于4 ℃，用时加入20%FBS，1 ng/ml bFGF。 1.5 培养皿处理 涂布3种不同的基质：培养前一天加入1 ml 1%明胶于35mm塑料培养皿，置于37 ℃培养箱过夜，接种前用D-Hanks液漂洗2次；接种前4 h，加入鼠尾
(100μM)15高糖+D药物(100μM)+B药物（1mM） 免疫沉淀过程实验试剂：A抗体（santa cruz，IP浓度 1:100）；B抗体（santa cruz，IP浓度 1:100）；Protein A+G Agarose（上海维景生物）蛋白样品的准备：1).对于10厘米细胞培养皿中的贴壁细胞，吸除细胞培养液，PBS洗涤一次，然后加入500微升至2毫升细胞裂解液裂解细胞。可以使用嘉美生物Western及IP细胞裂解液或各种RIPA裂解液等进行细胞的裂解。 2).对于组织样品参考贴壁细胞使用
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