- ¥780 - 2200
- LMAI Bio
- LM-5913R
- 进口/国产
- 2025年12月02日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-5913R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Calreticulin
- 抗体名：
钙网蛋白抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
- 免疫原：
KLH conjugated synthetic peptide derived from human Calreticulin:101-200/417
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
| 英文名称 | Calreticulin |
| 中文名称 | 钙网蛋白抗体 |
| 别 名 | Autoantigen RO; CALR; CALR protein; CALR_HUMAN; Calregulin; Calreticulin; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA. |
 |
Specific References (2) | bs-5913R has been referenced in 2 publications. [IF=5.69] Tanaka, Mamoru, et al. "Immunogenic cell death due to a new photodynamic therapy (PDT) with glycoconjugated chlorin (G-chlorin)." Oncotarget (2016). IHC-P ; Mouse. PubMed:27363018 [IF=1.58] Chen, Beilei, et al. "Calreticulin Binds to Fas Ligand and Inhibits Neuronal Cell Apoptosis Induced by Ischemia-reperfusion Injury." BioMed Research International. Human. PubMed:26583143 |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 信号转导 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 细胞定位 | 细胞浆 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calreticulin:101-200/417 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Subunit: Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 (By similarity). Interacts with NR3C1 and TRIM21. Interacts with GABARAP. Subcellular Location: Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Similarity: Belongs to the calreticulin family. SWISS: P27797 Gene ID: 811 Database links: Entrez Gene: 41166 Fruit fly (Drosophila melanogaster) Entrez Gene: 811 Human Entrez Gene: 12317 Mouse Entrez Gene: 100009050 Rabbit Entrez Gene: 64202 Rat Omim: 109091 Human SwissProt: P29413 Fruit fly (Drosophila melanogaster) SwissProt: P27797 Human SwissProt: P14211 Mouse SwissProt: P15253 Rabbit SwissProt: P18418 Rat Unigene: 2457 Fruit fly (Drosophila melanogaster) Unigene: 515162 Human Unigene: 1971 Mouse Unigene: 467043 Mouse Unigene: 974 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Calreticulin (bs-5913R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 49 kD  Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Calreticulin Polyclonal Antibody, Unconjugated(bs-5913R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-Calreticulin Antibody, conjugated (bs-5913R-AF488) at 1:200 overnight at 4°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.  Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Calreticulin Polyclonal Antibody, FITC conjugated (bs-5913R-FITC) 1:100, 40 minutes at 37°C.  Cell: Hela Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-5913R) on Hela(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody . |
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文献和实验三句话读懂一篇 CNS：睡眠太多或太少，都会增加中风风险；筛选高质量精子，科学家开发了新方法
ecto-calreticulin on ER-stressed cells。 该研究发现 NKp46 能够识别外化钙网蛋白 (ecto-CRT)，在内质网应激时从内质网 (ER) 转移到细胞膜。其中，ecto-CRT 对 NKp46 的识别能够有效地控制小鼠 B16 黑色素瘤和肺癌，并增强肿瘤浸润 NK 细胞的细胞因子分泌。 图 1：来源 Nature 2. Nature：PM2.5 促进肺癌发生的机制 PM 小于 2.5 的细颗粒可以进入肺部深处，引起多种疾病。 2023 年 4 月 5 日
MHCI 类分子荷肽 I类分子α链和β2m在糙面内质网中合成后被转运到光面内质网。α链在到达内质网后立即与伴随蛋白结合。参与I类分子加工的伴随蛋白很多，主要的有Cx、热休克蛋白(HSP)和钙网蛋白(calreticulin)。 不同的HSP分子是以亚基分子质量(kDa)来命名的，例如，HSP70是亚基分子质量为70 kDa的蛋白质。目前发现的HSP已有近30种，分为4组，即HSP70家族、HSP60家族、HSP90家族和低
点相当于一个自噬体，可以通过计数来评价自噬活性的高低。 3. 利用 Western Blot 检测 LC3-II/I 比值的变化以评价自噬形成 自噬形成时，胞浆型 LC3（即 LC3-I）会酶解掉一小段多肽，转变为（自噬体）膜型（即 LC3-II），因此，LC3-II/I 比值的大小可估计自噬水平的高低。 LC3 抗体对 LC3-II 有更高的亲和力，会造成假阳性。方法 2 和 3 需结合使用，同时需考虑溶酶体活性的影响。 4. MDC 染色 MDC 染色
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