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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-5810R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Lactoferrin
- 抗体名:
乳铁蛋白抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human Lactoferrin:611-710/710
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2ug/test IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
50ul 100ul 200ul
| 英文名称 | Lactoferrin |
| 中文名称 | 乳铁蛋白抗体 |
| 别 名 | CKRX; GIG12; Growth inhibiting protein 12; HLF2; Homo sapiens chemokine receptor X; Kaliocin 1; Lactoferrin; Lactoferroxin A; Lactoferroxin B; Lactoferroxin C; Lactoferroxin-C; Lactotransferrin; LF; LTF; Neutrophil lactoferrin; Talalactoferrin alfa; Talalactoferrin; TRFL_HUMAN. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2ug/test IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 76kDa |
| 细胞定位 | 细胞核 细胞浆 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Lactoferrin:611-710/710 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Function: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Isoform DeltaLf: transcription factor with antiproliferative properties and inducing cell cycle arrest. Binds to DeltaLf response element found in the SKP1, BAX, DCPS, and SELH promoters. Subunit: Monomer. Found in a complex with LTF, CLU, EPPIN and SEMG1. Subcellular Location: Isoform 1: Secreted. Isoform DeltaLf: Cytoplasm. Nucleus. Post-translational modifications: Isoform DeltaLf: Ubiquitinated at Lys-379 and Lys-391. Similarity: Belongs to the transferrin family. Contains 2 transferrin-like domains. SWISS: P02788 Gene ID: 4057 Database links: Entrez Gene: 4057 Human Entrez Gene: 17002 Mouse Entrez Gene: 301034 Rat Omim: 150210 Human SwissProt: P02788 Human SwissProt: P08071 Mouse Unigene: 529517 Human Unigene: 282359 Mouse Unigene: 218647 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: Lactoferrin protein (Human) Lysate at 100 ng Primary: Anti-Lactoferrin (bs-5810R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 85 kD ![]() Sample: Breast milk (Human) Lysate at 40 ug Primary: Anti-Lactoferrin (bs-5810R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 76 kD ![]() U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Antibody(bs-5810R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange). |
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文献和实验哪些组织以获取全长cDNA、进行原位杂交以了解更详细的空间表达信息等等。 利用RNA芯片只需要3个小时左右即可得到完整的结果,灵敏度高,即使是以往只有同位素能检测出来的低丰度的基因也能够被检测出来(例如占mRNA不到0.001%的乳铁传递蛋白(Lactoferrin)mRNA)。RNA芯片上的mRNA经过6个不同的看家基因进行严格的均质化(Normalization),足以保证根据实验结果对某个特定的mRNA存在与否及相对丰度能够得出准确的结论。
测定和校准PH. (二)蛋白质 泪液中蛋白质含量较高,在刺激后的泪液中占0.30.7%.泪液中至少有20种蛋白质。故泪液蛋白有其特殊性。其中泪白蛋白、溶菌酶和乳铁蛋白为泪液的主要蛋白质,占泪总蛋白的3545%,全部来自泪腺。其含量高低与泪腺功能有关。 1.抗微生物蛋白质泪中含有多种抗微生物蛋白质,主要有溶菌酶、乳铁蛋白、补体和SIGA、IGG、IGM型抗体等。 (1)溶菌酶:人泪溶菌酶浓度很高,约为血清溶菌酶浓度的150200倍。溶菌酶减低见于:①
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