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phospho-PIK3R2 (Tyr467)磷酸化磷脂酰肌

醇激酶p85β抗体
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  • ¥1580
  • LMAI Bio
  • LM-5582R
  • 进口/国产
  • 2025年12月18日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
  • Rabbit
  • Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-5582R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      phospho-PIK3R2 (Tyr467)

    • 抗体名

      磷酸化磷脂酰肌醇激酶p85β抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human PIK3R2 around the phosphorylation site of Tyr467:EE(P-Y)TR

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      100ul

    phospho-PIK3R2 (Tyr467)磷酸化磷脂酰肌醇激酶p85β抗体
    英文名称 phospho-PIK3R2 (Tyr467)
    中文名称 磷酸化磷脂酰肌醇激酶p85β抗体
    别    名 PIK3R2(phospho Y467); p85 antibody p85 beta; p85-BETA; P85B; P85B_HUMAN; Phosphatidylinositol 3 kinase; Phosphatidylinositol 3 kinase regulatory beta subunit; Phosphatidylinositol 3 kinase regulatory subunit beta; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase regulatory beta subunit; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphoinositide 3 kinase regulatory subunit 2 (beta); Phosphoinositide 3 kinase regulatory subunit 2; Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta); Phosphoinositide 3 kinase regulatory subunit polypeptide 2; Phosphoinositide 3 kinase, regulatory subunit 2 (beta); PI3 kinase p85 beta subunit; PI3 kinase p85 subunit beta; PI3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PI3K; PI3K regulatory subunit beta; PIK3R 2; PIK3R2; polypeptide 2 (p85 beta); PtdIns 3 kinase p85 beta; PtdIns-3-kinase p85-beta; PtdIns-3-kinase regulatory subunit beta; PtdIns-3-kinase regulatory subunit p85-beta.  PI 3-Kinase p85β; PI 3-Kinase p85-β; PI 3-Kinase p85 β;
    规格价格 100ul/1580元 购买        大包装/询价
    说 明 书 100ul  
    产品类型 磷酸化抗体 
    研究领域 免疫学  信号转导  激酶和磷酸酶  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 82kDa
    细胞定位 细胞核 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human PIK3R2 around the phosphorylation site of Tyr467:EE(P-Y)TR 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012]

    Function:
    Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.

    Subunit:
    Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated). Interacts with NYAP1, NYAP2 and MYO16.

    Post-translational modifications:
    Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ.

    Similarity:
    Belongs to the PI3K p85 subunit family. 
    Contains 1 Rho-GAP domain. 
    Contains 2 SH2 domains. 
    Contains 1 SH3 domain.

    SWISS:
    O00459

    Gene ID:
    5296

    Database links:

    Entrez Gene: 5296 Human

    Omim: 603157 Human

    SwissProt: O00459 Human

    Unigene: 371344 Human



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 产品细节图片1
    Sample: 293T Cell (Human) Lysate at 40 ug
    Primary: Anti-phospho-PIK3R2(Tyr467) (bs-5582R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 82 kD
    Observed band size: 82 kD

    产品细节图片2
    Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIK3R2 (Tyr467)) Polyclonal Antibody, Unconjugated (bs-5582R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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    图标文献和实验
    相关实验
    • Using Phospho‐Motif Antibodies to Determine Kinase Substrates

      the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation

    • 运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白

      results of phosphorylated proteins磷酸化的试剂盒中的抗体是经过大量筛选,选取了高特异性和低背景的抗体。p-P38MAPK抗体是基于P38的磷酸化Thr180到Tyr182这个位点多肽片段所制备。P-JNK抗体是基于人的Thr183和Tyr185.这个抗体可以同时识别P-JNK1和P-JNK2.我们在运用Cell Based Elisa P38和JNK两个试剂盒做检测的同时,又用传统的WB方式来验证这个结果。经过反复验证,都得出了同样的结果, Cell Based

    • Nature 子刊:林圣彩、宋尔卫等建立脂质生成与乳腺癌的直接联系

      依赖的方式磷酸化,这些数据清楚地表明,LPIN1 是 Src 真正的底物。那么磷酸化的 LPIN1 是否直接影响 MDA-MB-231 和 MDA-MB-468 细胞中甘油脂的合成呢?研究发现,当在无血清培养基中培养时,生长因子的添加可显著加快了这两种乳腺癌细胞系中甘油二酯 (DAG)、甘油三酯 (TAG)、磷脂酰乙醇胺 (PE) 和磷脂酰丝氨酸 (PS) 的合成速度,磷脂酰胆碱 (PC) 合成率的平均值也有增加趋势提高。然而,这种增加的效应在 LPIN1 联合突变细胞中却消失了。乳腺癌细胞的增殖

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