EPAC 5376753

Imaging cAMP Dynamics in the Drosophila Brain with the Genetically Encoded Sensor Epac1-Camps

, we describe a detailed protocol for the monitoring of neuronal cAMP dynamics during bath application of neurotransmitters and neuromodulators using the genetically encoded cAMP sensor Epac1-camps and scanning laser confocal microscopy.

Exchange Protein Directly Activated by Cyclic AMP-1-Regulated Recruitment of CCAAT/Enhancer-Binding Proteins to the Suppressor of Cytokine Signaling-3

. However, it is now clear that alternative intracellular cAMP sensors, of which the exchange protein directly activated by cAMP (Epac) proteins have been studied most intensively, also initiate transcription of key genes in response to cAMP elevation. For example

BRET Approaches to Characterize Dopamine and TAAR1 Receptor Pharmacology and Signaling

to analyze dynamics of cAMP modulation via an EPAC biosensor as well as recruitment of β-arrestin2 to the D2 dopamine receptor. Combination of these approaches allows for a comparison of activity of pharmacological compounds on these signaling modalities