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货期:1-2天
- 供应商:
MedChemExpress LLC
- 规格:
25 pcs/50 pcs
| 规格: | 25 pcs | 产品价格: | ¥950.0 |
|---|---|---|---|
| 规格: | 50 pcs | 产品价格: | ¥1550.0 |
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蛋白酶抑制剂 Cocktail, 片剂 (EDTA-Free)
MCE 国际站:蛋白酶抑制剂 Cocktail, 片剂 (EDTA-Free)
蛋白酶抑制剂Cocktail(mini片剂)可以用于细胞或组织提取物用来增加蛋白稳定性。
Description & Advantages:
Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases. Generally, protease inhibitor cocktails are used in cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. MedChemExpress Protease Inhibitor Cocktail (EDTA free, mini-Tablet) is a blend of 5 pan-protease inhibitors for protection of protein integrity.
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文献和实验问:请教一个实验问题,PMSF,胃酶抑制剂以及DTT会不会影响HRP活性?要做ELISA,样品中不知道可不可以加复合蛋白酶抑制剂?请大牛指教!答:不会影响酶活性,因为蛋白酶抑制剂,抑制的是蛋白水解酶活性。而HRP不是蛋白酶类,而是过氧化酶。(不过dtt肯定会破坏HRP)。即使会影响酶活性,实际上做elisa也不影响,因为加样品和加hrp是分开的,不存在。 答:我在研究各种抑制剂对L-氨基酸氧化酶酶活性的抑制作用的时候,测定L-氨基酸氧化酶活性的体系含有HRP。我们首先用各种抑制剂对HRP水解
before use. Cocktail solutions are stored at -20 degrees and are good for approximately 6 months. I. Protease Inhibitor Cocktail The Protease inhibitor cocktail is used as 100X for extract preps and as 1000X for column elution buffers
Xeno-Free Culture of Human Pluripotent Stem Cells
substratum: (1) TeSR2™ with human recombinant laminin (LN-511); (2) NutriStem™ with LN-511; (3) RegES™ with human foreskin fibroblasts (hFFs); (4) KO-SR Xeno-Free™/GF cocktail with CELLstart™ matrix.
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