- ¥3450
- Cloud-Clone Corp. (美国)
- 0.156-10ng/mL
- SEJ829Hu
- 2025年07月14日
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- 详细信息
- 技术资料
- 检测范围：
0.156-10ng/mL
- 检测方法：
酶联免疫吸附试验法
- 应用：
ELISA
- 样本：
Tissue homogenates and other biological fluids.
- 供应商：
上海研卉生物科技有限公司
- 库存：
1周
- 规格：
96T
磷酸肌醇-3-激酶催化亚基β肽(PIK3Cβ)检测试剂盒
ELISA Kit for Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb)
产品编号 SEJ829Hu 适用生物 Homo sapiens (Human，人)
品牌商 Cloud-Clone Corp. (美国)
检测范围 0.156-10ng/mL 灵敏度 0.059ng/mL
样本类型 Tissue homogenates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
ELISA Kit for Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb)
产品编号 SEJ829Hu 适用生物 Homo sapiens (Human，人)
品牌商 Cloud-Clone Corp. (美国)
检测范围 0.156-10ng/mL 灵敏度 0.059ng/mL
样本类型 Tissue homogenates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
ELISA Kit for Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEJ829Hu |
| Sample type | Tissue homogenates and other biological fluids. |
| Format | 96-well strip plate |
| Assay length | 4.5 hours |
| Detection range | 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.059ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb).
No significant cross-reactivity or interference between Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) and analogues was observed.
No significant cross-reactivity or interference between Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
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磷酸肌醇-3-激酶催化亚基β肽(PIK3Cβ)检测试剂盒
¥3450
