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- 详细信息
- 文献和实验
- 技术资料
- 检测范围:
0.156-10ng/mL
- 检测方法:
酶联免疫吸附试验法
- 应用:
ELISA
- 样本:
Tissue homogenates and other biological fluids.
- 供应商:
上海研卉生物科技有限公司
- 库存:
1周
- 规格:
96T
ELISA Kit for Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb)
产品编号 SEJ829Hu 适用生物 Homo sapiens (Human,人)
品牌商 Cloud-Clone Corp. (美国)
检测范围 0.156-10ng/mL 灵敏度 0.059ng/mL
样本类型 Tissue homogenates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
| Organism species | Homo sapiens (Human) |
| Product No. | SEJ829Hu |
| Sample type | Tissue homogenates and other biological fluids. |
| Format | 96-well strip plate |
| Assay length | 4.5 hours |
| Detection range | 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.059ng/mL. |
No significant cross-reactivity or interference between Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) and analogues was observed.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
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文献和实验Mol Cell:杨薇等揭示 DNA-PK 的激活机制以及自磷酸化对 NHEJ 通路的重要作用
and Rad3-Related)三种激酶。 其中,DNA-PK 是非同源末端连接(Non-homologous end joining,NHEJ)通路中的关键蛋白激酶,由 DNA-PKcs(DNA-PK 催化亚基)、Ku70/80 和 DNA 末端组成,其全酶装配会进一步招募 NHEJ 相关的连接酶和修复蛋白,包括 DNA 连接酶 IV、XRCC4(X-ray cross complementing protein 4)、XLF(XRCC4-like factor)和 Artemis 等。 由于激酶
,能与配体直接结合,对 Hh 信号起负调控作用。受体 Smo 由原癌基因 Smothened 编码,与 G 蛋白偶联受体同源,由 7 个跨膜区的单一肽链构成,N 端位于细胞外,C 端位于细胞内,跨膜区氨基酸序列高度保守,C 末端的丝氨酸与苏氨酸残基为磷酸化部位,蛋白激酶催化时结合磷酸基团。该蛋白家族成员只有当维持全长时才有转录启动子的功能,启动下游靶基因的转录。
趋化因子受体信号传递 趋化因子受体与相应配体结合后引发胞内复杂的信号转导级联反应。第一步是受体与其高亲和力配体的结合,引起构象变化导致受体关联的G蛋白异三聚体分解为α和βγ亚基,α和βγ亚基作为第二信使激活随后的一系列效应酶,如磷脂酶C和蛋白激酶。 主要的趋化因子受体、配体及其分布 磷脂酶C可引发磷酸肌醇C信号通路和胞内Ca2+浓度的增加。这个信号转导级联反应不仅可以调节肌动蛋白依赖的细胞
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