手机验证
100
Virus DNA/RNA Extraction Kit II
VR050/VR100/VR300
2年
科昊佳生物
RT
100T
英文名 | 中文名 | 品牌 | 货号 | 规格 |
Virus DNA/RNA Extraction Kit II | 病毒核酸纯化试剂盒 | Geneaid Biotech | VR100 | 100T |
Virus DNA/RNA Extraction Kit II VR050/VR100/VR300
The Viral Nucleic Acid Extraction Kit II was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. The efficient glass fiber spin column system is optimized for nucleic acid purification from a wide variety of both DNA and RNA viruses such as HBV, CMV, HCV, HIV, and HTLV. 101-109 copies of viral DNA/RNA can be purified from up to 200 μl samples within 20 minutes. The purified viral DNA/RNA can be used directly in qPCR and qRT-PCR assays.
Advantages (Cat. # VR050, VR100, VR300)
High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number!
Purify virus DNA or virus RNA in 20 minutes!
Sample Volume: up to 200 μl samples of plasma, serum, body fluids, supernatant of viral cell cultures
Spin Columns: glass fiber membrane optimized for virus DNA and virus RNA purification
Individually packaged virus spin columns and collection tubes, certified RNase and DNase-free
Elution Volume: 50 μl
Storage: dry at room temperature (15-25oC)
Applications
RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS)
Components
VB Lysis Buffer
AD Buffer
W1 Buffer
Wash Buffer
RNase-free Water
VB Columns
2 ml Collection Tubes
Quality Control
The quality of Viral Nucleic Acid Extraction Kit II is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating viral DNA/RNA from a 200 μl serum sample.
Viral Nucleic Acid Extraction Kit II Functional Test Data
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Figure 2. Hepatitis A Virus (HAV) RNA was extracted using the Geneaid Viral Nucleic Acid Extraction Kit II. The purified RNA was analyzed by electrophoresis on a 1% agarose gel. 2: HAV from 10X diluted serum Product Original Serum 10XDiluted Serum 100XDiluted Serum Virus DNA/RNA Kit 2.17 x 107 4.47 x 106 8.48 x 105 |
Figure 3. HBV (DNA), HCV (RNA), HIV (RNA), and HTLV (RNA) were purified from 200 μl of positive clinical serum samples using the Viral Nucleic Acid Extraction Kit II. Real-time qPCR and 1-step qRT-PCR reactions were then conducted using the ABI 7300 Sequence Detection System (3 replications of each copy number). Serum samples containing various amounts of DNA/RNA viruses ranging from 10E1 to 10E6 copies/ml were successfully detected and identified. The low Ct values indicate a high number of target nucleic acid in the sample.
Publications
Journal Article 1
Journal Article 2
Journal Article 3
Journal Article 4
Journal Article 5
Related Virus DNA/RNA Purification Products
Product Name |
RXNS |
Catalogue Number |
Viral Nucleic Acid Extraction Kit II (up to 200 μl sample) |
50, 100, 300 |
VR050/100/300 |
Viral Nucleic Acid Extraction Kit III (up to 1 ml sample) |
50, 100, 300 |
VI050/100/300 |
Plant Virus RNA Kit |
50, 100 |
PVR050/100 |
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VR100 英文说明书(protocol).pdf 附 (下载 2 次)