- ¥780 - 2200
- LMAI Bio
- LM-4975R
- 进口/国产
- 2026年01月23日
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Chicken, Pig, Cow,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-4975R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
CCK8
- 抗体名：
胆囊收缩素8抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Chicken, Pig, Cow,
- 免疫原：
KLH conjugated synthetic peptide derived from human CCK8:96-103/115
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
| 英文名称 | CCK8 |
| 中文名称 | 胆囊收缩素8抗体 |
| 别 名 | CCK 8; CCK; CCK8; CCK-8, Cholecystokinin 8; Cholecystokinin;Cholecystokinin preproprotein; Cholecystokinin8; Cholecystokinins precursor; MGC117187; Procholecystokinin precursor. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 信号转导 生长因子和激素 内分泌病 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Pig, Cow, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 12.6kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CCK8:96-103/115 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Cholecystokinin is a brain/gut peptide. In the gut, it induces the release of pancreatic enzymes and the contraction of the gallbladder. In the brain, its physiologic role is unclear. The cholecystokinin pro-hormone is processed by endo- and exo-proteolytic cleavages. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]. Function: Cholecystokinin (CCK) is one of the most important hormone regulators of the digestive process. The peptide is concentrated in the proximal small intestine (CCK cells) and is secreted into the blood in response to the ingestion of proteins and fats, where its actions include the stimulation of pancreatic secretion, the modulation of insulin output, the regulation of gastric emptying, and gallbladder contraction. In the central nervous system CCKergic fibres are widespread, being particularly abundant in the cerebral cortex. In some mesolimbic neurons projecting to the nucleus accumbens, CCK is colocalised with dopamine and it has been suggested that CCK might facilitate the function of dopamine in events such as stimulusreward associative behaviour. Secretion of CCK from the murine tumour cell line STC1 has been shown to be stimulated by pituitary adenylate cyclase activating protein (PACAP) and has raised the possibility that PACAP may function as a neuromodulator of CCK release from gut endocrine cells in vivo. Subunit: Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain. Subcellular Location: Secreted. Tissue Specificity: The shortest form (CCK8) is predominantly found in the brain, whereas the larger ones are found in the intestine. Similarity: Belongs to the gastrin/cholecystokinin family. SWISS: P06307 Gene ID: 855 Database links: Entrez Gene: 885 Human Omim: 118440 Human SwissProt: P06307 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CCK8 Polyclonal Antibody, Unconjugated(bs-4975R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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文献和实验从猪小肠提取物中分离出来的、由33个氨基酸组成的多肽。具刺激胆囊收缩和兴奋胰酶分泌的作用。于1928年、1943年被证明并提纯。天然CCK化学结构有多种，有CCK-33，CCK-39，CCK-58，以及从山羊和人小脑中分离出一种8肽CCK等。含CCK的细胞存在于哺乳动物十二指肠和空肠粘膜，其细胞与人肠I细胞同。1978年有人发现，CCK还存在于中枢神经系统，且含量大于小肠内含量，存在于皮层额叶、皮层梨状区、尾核、海马、丘脑、下丘脑、小脑和间脑。CCK在血中很快降解，其半衰
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人胆囊收缩素 ( CCK-8 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 CCK-8 单抗包被于酶标板上，标准品和样品中的 CCK-8与单抗结合，加入生物素化的抗人 CCK
技术原理与方法｜超级色差校正物镜 PLAPON60XOSC 在脑组织四重免疫荧光分析中的应用
染色（Alexa Fluor405，蓝色）；CB1 染色（Alexa Fluor488，绿色）；VIP 染色（Cy3，红色）；DGLα染色（Alexa Fluor647，白色）。 GABAergic 篮状细胞有两种表达大麻素受体 CB1 并控制锥体细胞躯体的亚型，一种亚型表达胆囊收缩素（CCK），另一种表达血管活性肠肽（VIP）。这种四重免疫荧光染色显示，在高表达 CB1（绿色）和 VIAAT（蓝色）的阳性神经末梢（红色）周围缺乏 DGLα（白色），其表明 VIP 阳性神经末梢不参与侵入性突触
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