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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-4910R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
MCSF
- 抗体名:
巨噬细胞克隆刺激因子抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
- 免疫原:
KLH conjugated synthetic peptide derived from human MCSF/M-CSF:201-300/554 <Extracellular>
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
50ul 100ul 200ul
| 英文名称 | MCSF |
| 中文名称 | 巨噬细胞克隆刺激因子抗体 |
| 别 名 | M-CSF; Macrophage Colony Stimulating Factors; Colony stimulating factor 1 (macrophage); Colony stimulating factor 1; Colony stimulating factor macrophage specific; CSF 1; CSF1; Csfm; Lanimostim; M CSF; Macrophage Colony Stimulating Factor 1; MCSF; MGC31930; CSF1_HUMAN. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 发育生物学 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 57kDa |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MCSF/M-CSF:201-300/554 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Four distinct colony-stimulating factors (CSFs) that promote survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte macrophage CSF (GMCSF), granulocyte CSF (GCSF), macrophage CSF (MCSF), and Interleukin-3 (IL-3, Multi CSF). Both GMCSF and IL-3 are multipotential growth factors, stimulating proliferation of progenitor cells from more than one hematopoietic lineage. In contrast, GCSF and MCSF are lineage restricted hematopoietic growth factors, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors. Function: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Subunit: Homodimer or heterodimer; disulfide-linked. Interacts with CSF1R. Subcellular Location: Cell membrane; Single-pass membrane protein. Processed macrophage colony-stimulating factor 1: Secreted, extracellular space. Post-translational modifications: N-glycosylated. The predominant soluble form is a chondroitin sulfate-containing proteoglycan. DISEASE: Note=A defect in Csf1 is the cause of osteopetrosis. Osteopetrotic mice (op/op) are severely deficient in mature macrophages and osteoclasts, display failed tooth eruption, and have a restricted capacity for bone remodeling. SWISS: P09603 Gene ID: 1435 Database links: Entrez Gene: 1435 Human Omim: 120420 Human SwissProt: P09603 Human Unigene: 591402 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. -CSF是促使造血细胞分化成粒细胞与巨噬细胞克隆的重要细胞因子,对正常血细胞的形成与造血细胞 的存活、分化、正常造血功能 的维持具有重要作用,直接与间接增加lak细胞的活性,在人体 的细胞因子网络中占有重要 的地位,目前广泛应用于化.放疗 的辅助治疗中。 目前研究主要应用在免疫缺陷性疾病及病毒性疾病:慢性肝病,尤其是与病毒相关的疾病,因其发病过程涉及免疫细胞与造血系统,有可能成为gm-csf(粒细胞-巨噬细胞克隆刺激因子)应用的新领域。人和小鼠天然M-CSF为糖蛋白,由二硫键连接的同源双体,分子量40~90kDa。人和小鼠M-CSF分子这个区域结构高度保守,其同源性达80%。 |
| 产品图片 | ![]() Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-MCSF (bs-4910R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 57 kD Observed band size: 57 kD |
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文献和实验碱性粒细胞的增殖,阻止肥大细胞发生程序性细胞死亡。 (二)GM-CSF 1977年Burgess等从小鼠肺条件培养液中发现一种能刺激粒细胞和巨噬细胞形成集落的因子,命名为粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)。1984年和1985年小鼠和人GM-CSF的cDNA分别克隆成功。 1.GM-CSF的产生 T细胞、B细胞、巨噬细胞、肥大细胞、内皮细胞、成纤维细胞等均可产生GM
另外,尚有包括本文介绍的多项基于该技术的 COVID-19 候选疫苗正在研制中。基于 RNA 技术的策略能否给新冠肺炎带来新的曙光,一起拭目以待。 推荐阅读: 北大谢晓亮及合作者 Cell 发表新冠病毒中和抗体研究结果,单细胞测序技术助力新冠病毒抗体研发 Science 重磅 | 高福院士等学者分离鉴定出抑制新冠病毒感染的人源单克隆抗体 Moderna 发布疫苗最新临床结果,所有受试者都产生抗体! 参考文献向上滑动阅览 1.
Cancer Immunol Res:胃癌免疫治疗新突破!南京大学魏嘉教授团队揭示胃癌免疫微环境全新调控靶点
组织,与各种免疫标志物(PDL1、TMB、MSI 等)存在显著相关性,并预示患者较差的预后。以上临床数据提示 DKK1 可能是胃癌患者免疫治疗应答及预后的一种潜在标志物。 为了探索胃癌 DKK1 表达在体内的影响,研究人员使用了包括小鼠皮下、原位、腹腔转移、免疫缺陷在内的多个胃癌模型,研究阻断 DKK1 能否抑制肿瘤生长。结果显示给予 DKK1 单克隆抗体 mDKN-01 可有效控制多个胃癌模型的肿瘤进展,并展现出较好的生物安全性。此外,研究人员发现,阻断 DKK1 还可显著改善小鼠肿瘤局部的免疫微环
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