- ¥1380 - 2200
- LMAI Bio
- LM-4895R
- 进口/国产
- 2026年01月09日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-4895R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
PIRH2
- 抗体名：
泛素连接酶抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 免疫原：
KLH conjugated synthetic peptide derived from human PIRH2:31-130/261
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
100ul 200ul
| 英文名称 | PIRH2 |
| 中文名称 | 泛素连接酶抗体 |
| 别 名 | Androgen receptor N terminal interacting protein; Androgen receptor N-terminal-interacting protein; ARNIP; CH-rich-interacting match with PLAG1; CHIMP; E3 ubiquitin-protein ligase Pirh2; hARNIP; hPirh2; p53 induced protein with a RING H2 domain; p53-induced RING-H2 protein; PIRH2E; PIRH2F; PRO1996; RCHY1; Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase; RING finger and CHY zinc finger domain-containing protein 1; RING finger protein 199; RNF199; ZFP 363; Zinc finger protein 363; ZN363_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 30kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PIRH2:31-130/261 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Pirh2 has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. The protein physically interacts with p53 and the resulting degradation of p53 renders Pirh2 an oncogenic protein as the loss of p53 function contributes to malignant tumor development. The gene encoding for the protein maps to chromosome 4q21.1 and transcription of this gene is regulated by p53. Pirh2 expression decreases the level of p53 and a decrease of endogenous Pirh2 expression ups p53 levels. Pirh2 is therefore considered, together with MDM2, to be acting as a negative regulator of p53 function. Function: Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Increases AR transcription factor activity (By similarity). Contributes to the regulation of CDKN1B and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Subunit: Monomer and homodimer. Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, CDKN1B, COPE, UBE2D2 and GORAB/NTKLBP1 (By similarity). Subcellular Location: Nucleus (By similarity). Nucleus speckle (By similarity). Cytoplasm (By similarity). Tissue Specificity: Detected in testis, liver, kidney and heart. Post-translational modifications: Subject to ubiquitination and proteasomal degradation (By similarity). Similarity: Contains 1 CHY-type zinc finger. Contains 1 CTCHY-type zinc finger. Contains 1 RING-type zinc finger. SWISS: Q96PM5 Gene ID: 25898 Database links: Entrez Gene: 771726 Chicken Entrez Gene: 540733 Cow Entrez Gene: 25898 Human Entrez Gene: 68098 Mouse Entrez Gene: 289508 Rat Omim: 607680 Human SwissProt: Q96PM5 Human SwissProt: Q9CR50 Mouse Unigene: 48297 Human Unigene: 159453 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Pirh2通过负反馈调控p53的转录激活和细胞生长抑制功能； Pirh2蛋白是一个新发现的泛素蛋白连接酶，受到p53的诱导调控表达，可促进p53蛋白的泛素化降解。目前多用于肺癌的研究。 |
| 产品图片 |  Sample:Kidney(Mouse)Lysate at 40 ug Primary: Anti-PIRH2(bs-4895R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 30kD Observed band size: 30kD  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PIRH2 Polyclonal Antibody, Unconjugated(bs-4895R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PIRH2 Polyclonal Antibody, Unconjugated(bs-4895R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 泛素连接酶 (UBPL)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E3/UBPL 单抗包被于酶标板上，标准品和样品中的 UBPL与单抗结合，加入生物素化的抗人
Cell Metab：西京医院王琳教授团队报道非酒精性脂肪肝炎新靶点
Metabolism 上在线发表了题为 Tripartite motif 16 ameliorates nonalcoholic steatohepatitis by promoting the degradation of phospho-TAK1 的研究成果 (4)。该研究发现 E3 泛素连接酶 TRIM16 通过促进 p-TAK1 的泛素化依赖性降解，改善 NASH 进展过程中的脂质积累和炎症。 图片来源：Cell Metabolism研究内容肝细胞的脂肪毒性诱导 TRIM16 的表达脂肪毒性是脂质过度积累
糖基化、糖基化和磷酸化糖基化。3、Ubiquitination：泛素是一类分子量为 8 kDa 的多肽，由 76 个氨基酸组成，通过泛素 C 末端的甘氨酸连接到靶蛋白赖氨酸的 ε-NH2 上。在最初的单泛素化事件之后，可能形成泛素聚合物，然后 26S 蛋白酶体识别多聚泛素化蛋白，26S 蛋白酶体催化泛素化蛋白的降解和泛素的再循环。以下实验提供了检测泛素化蛋白的方法示例。 图示：HeLa 细胞裂解液中泛素的检测。进行 Weste blot 分析，比较 4 种检测 HeLa 细胞裂解液中泛素蛋白的方法
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