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SLAMF7抗体

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  • ¥1380 - 2200
  • LMAI Bio
  • LM-2544R
  • 进口/国产
  • 2026年01月12日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
  • Rabbit
  • Mouse, Rat, Cow,
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    • 详细信息
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-2544R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      SLAMF7

    • 抗体名

      SLAMF7抗体

    • 宿主

      Rabbit

    • 适应物种

      Mouse, Rat, Cow,

    • 免疫原

      KLH conjugated synthetic peptide derived from human SLAMF7:51-160/335 <Extracellular>

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      100ul  200ul

    SLAMF7抗体
    英文名称 SLAMF7
    中文名称 SLAMF7抗体
    别    名 19A; 19A24 protein; CD2 like receptor activating cytotoxic cells; CD2 subset 1; CD 319; CD319; CD319 antigen; CRACC; CS1; Membrane protein FOAP 12; Novel Ly9; Novel LY9 (lymphocyte antigen 9) like protein; Protein 19A; SLAM family member 7; SLAMF 7; SLAMF7.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 细胞生物  免疫学  淋巴细胞  t-淋巴细胞  b-淋巴细胞  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Mouse, Rat, Cow, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 33kDa
    细胞定位 细胞膜 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human SLAMF7:51-160/335 <Extracellular>
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain. Isoform 1 mediates NK cell activation through a SAP-independent extracellular signal-regulated ERK-mediated pathway. It may play a role in lymphocyte adhesion. Isoform 3 does not mediate any activation. SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro). SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. The isoform 3 is expressed at much lower level than isoform 1. There are three named isoforms.

    Function:
    Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. 
    Isoform 3 does not mediate any NK cell activation.

    Subunit:
    Isoform 1 binds to SH2D1A when its cytoplasmic tail is phosphorylated in the presence of FYN (in vitro).

    Subcellular Location:
    Membrane; Single-pass type I membrane protein.

    Tissue Specificity:
    Expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. Isoform 3 is expressed at much lower level than isoform 1

    Similarity:
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

    SWISS:
    Q9NQ25

    Gene ID:
    57823

    Database links:

    Entrez Gene: 57823 Human

    Omim: 606625 Human

    SwissProt: Q9NQ25 Human

    Unigene: 517265 Human



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 产品细节图片1
    Sample: 
    Lymph node (Mouse) Lysate at 40 ug
    Primary: Anti-SLAMF7 (bs-2544R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 33 kD
    Observed band size: 58 kD

    产品细节图片2
    Blank control (black line): A431. 
    Primary Antibody (orange line): Rabbit Anti-SLAMF7/AF488 Conjugated antibody (bs-2544R-AF488) 
    Dilution: 1μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG-AF488.
    Protocol
    The cells were fixed with 4% paraformaldehyde for 10 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
    产品细节图片3
    Blank control (blue line): A431(black). 
    Primary Antibody (green line): Rabbit Anti-SLAMF7 antibody (bs-2544R) 
    Dilution: 3μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line):Goat anti-rabbit IgG-PE(Jackson lab)
    Dilution: 1μg /test. 
    Protocol
    The cells were fixed with 4% paraformaldehyde for 10 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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