- ¥320.58
- Sigma
- M9281
- 进口
- 2026年01月04日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
3-Methyladenine
- 供应商：
上海善然生物科技有限公司
- 保存条件：
低温
性质
Related Categories Autophagy, Autophagy Inhibitors, Core Bioreagents, Nucleoside Analogs, Nucleosides and Nucleotides,
更多...
Quality Level PREMIUM
InChI Key FSASIHFSFGAIJM-UHFFFAOYSA-N
mp ~300 °C (dec.)(lit.)
产品描述
包装
100, 500 mg in poly bottle
Preparation Note
This product is soluble in DMF (10 mg/ml, may require gentle heating), yielding a clear, colorless solution. It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/ml with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA are best prepared fresh.
Application
3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.
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文献和实验specificities. Isoschizomers with different sensitivity to methylation are indicated by "m ". DpnI requires the presence of N6 -methyladenine within the recognition sequence GATC. Single letter code: R = G or A; Y = C or T; W = A or T;
Typical transformation results
-----------------------HAPLOID Transformation results for ORF YER142C Gene = MAG1 Gene product = 3-methyladenine DNA glycosylase Previous disruption = viable
DNA Methyltransferases as Probes for Chromatin Structure in Yeast
the production of N6 -methyladenine (6me A) at GATC sites, were limited by the occurrence of a target site only once every ∼300 bp in native yeast sequences (1 ,2 ). This limited resolution of dam MTase, although possible to circumvent through the introduction
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