- ¥219.96
- sigma
- 进口
- M8410-500ML
- 2025年07月15日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
低温
- 英文名：
Mineral oil
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
- 规格：
500ML
矿物油
Mineral oil
light oil (neat), BioReagent, suitable for mouse embryo cell culture
产品名称
Analysis Note
This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.
Application
Recommended for overlaying mouse embryo cultures to prevent drying.
Caution
This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.
包装
1 L in poly bottle
5, 100, 500 mL in poly bottle
性质
| Related Categories | Mouse Embryo Culture, Reagents and Media Supplements, 特殊培养基系统, 细胞培养 |
| product line | BioReagent |
| form | light oil (neat) |
| refractive index | n |
| density | 0.84 g/mL at 25 °C(lit.) |
| suitability | suitable for mouse embryo cell culture |
| R2625-50MG | 维生素A |
| 415324-50G | N-异丙基丙烯酰胺 |
| A3411-10MG | 氨基蝶呤 |
| 52365-250G | CTAB |
| 62476-500G-F | 氯化锂 |
| PVP40-500G | PVP40 |
| E5134-5KG | EDTA-Na2 |
| T1503-5KG | Tris 碱 |
| A9539-250G | 琼脂糖 |
| G9752-500G | 阿拉伯树胶 |
| 95904-1KG-F | PEG 4000 |
| M3671-50G | 无水MES |
| P7545-25G | 胰酶 |
| DN25-100MG | 脱氧核糖核酸酶 I |
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