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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
GSH-coupled Agarose Affinity Matrix
- 保质期:
一年
- 供应商:
上海信裕生物科技有限公司
- 保存条件:
4℃保存,有效期一年;切勿冻结!
- 规格:
10 ml/50 ml
谷胱甘肽琼脂糖亲和基质是结合谷胱甘肽(GSH)的4%浓度的交联珠状琼脂糖基质,可用于结合和分离纯化带有谷胱甘肽硫转移酶(GST)等谷胱甘肽亲和性蛋白质分子,特别是重组表达的GST融合蛋白。
用途
· 带有GST标签的重组蛋白的纯化
· GST pull-down实验
特点
· 特异性高:很少与非GST融合蛋白相结合
· 高结合量:每毫升基质可结合5~10 mg重组GST融合蛋白*
· 高度灵活:可填装任意品牌的柱子
储存条件
4℃保存,有效期一年;切勿冻结!
主要成分
50%GSH-Agarose亲和基质悬液,PBS,20%乙醇
*注:基质的蛋白结合量因融合蛋白性质不同而异,通常每毫升基质可结合数百微克至数毫克蛋白。
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文献和实验Orientation of the Agarose Matrix by Pulsed Electric Fields
DNA molecules less than ~20 kilobase (kb) pairs in size are efficiently fractionated by unidirectional electrophoresis in agarose gels (1 ). The variation of DNA mobility with gel concentration can be described by the Ogston mechanism
On-Column Ligand/Receptor Derivatization Coupled to Affinity Capillary Electrophoresis
The coupling of on-column derivatization of small molecules to affinity capillary electrophoresis (ACE) has only been realized during the past 5 yr. In this technique, multiple zones of reagent(s) and ligand or receptor are injected
Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose
attempted affinity methods. Our laboratory has used Reactive Red-agarose in the purification of m-and μ-calpain from bovine heart and other tissues for many years (1 ,2 ). However, this method has not achieved widespread use due to reported variability
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