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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
EZ-Lyse Rapid Colony Identification Kit
- 保质期:
一年
- 供应商:
上海信裕生物科技有限公司
- 保存条件:
室温保存一年
- 规格:
100 rxn/1000 rxn
EZ-Lyse Rapid Colony Identification Kit是专为快速筛选阳性克隆质粒或检测克隆效率而特别设计的产品。该试剂盒可在连接产物转化、过夜培养后,在数分钟内从单个菌落中提取DNA样品,并直接上样进行琼脂糖电泳,通过与空载体比对质粒大小而判断载体中是否含有插入片段,以便快速挑选阳性克隆进行质粒提取、酶切、测序等后续实验。
用途
· 快速判断单个克隆是否含有插入片段
特点
· 快捷简便:30~40分钟完成质粒粗提和电泳检测,减少质粒提取的样品量,降低成本,简化操作
· 准确稳定:适合大量克隆的粗筛
· 避免菌落PCR常见的假阳性问题
产品组分
| 货号 | XY171-01 | XY171-10 |
| 规格 | 100rxn | 1000rxn |
| EZ-Lyse Buffer 1 | 1 ml | 10 ml |
| EZ-Lyse Buffer 2 | 1 ml | 10 ml |
室温保存一年
使用方法
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文献和实验Rapid Identification of Cloned HIV-1 Fragments
can be identified. An alternative but equally time-consuming approach is the procedure of colony filter hybridization (1 ,2 ). This procedure includes transferring bacterial colonies to a nitrocellulose or nylon membrane, denaturing plasmid DNA in situ
Rapid identification of differentially expressed genes by in situ screening of bacteria
The identification of differentially expressed genes is a key step in the understanding of specific molecular mechanisms. Various methods have been developed to search for differences in expression but most of them are time or money consuming
Fastfilter Plasmid Maxi Kit Spin Protocol
as inoculun, streak it onto an agar plate containing the appropriate antibiotic for single colony isolation. Then pick a single colony and inoculate the 2-5ml starter culture as described above. Lyse bacterial cells with alkaline-SDS Solution:
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