- ¥780 - 2200
- LMAI Bio
- LM-1948R
- 进口/国产
- 2025年12月23日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Pig, Cow, Rabbit,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-1948R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
WNT5A
- 抗体名：
信号通路Wnt5a抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Pig, Cow, Rabbit,
- 免疫原：
KLH conjugated synthetic peptide derived from human WNT5A:301-381/381
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
| 英文名称 | WNT5A |
| 中文名称 | 信号通路Wnt5a抗体 |
| 别 名 | wingless-related MMTV integration site 5A; hWNT 5A; hWNT5A; Protein Wnt 5a; Protein Wnt5a; Wingless type MMTV integration site family member 5A; Wnt-5a; WNT 5A protein precursor; WNT5A protein precursor; WNT5A_HUMAN. |
 |
Specific References (1) | bs-1948R has been referenced in 1 publications. [IF=3.31] Król, Magdalena, et al. "Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors." PloS one 9.1 (2014): e83995. WB ; Dog. PubMed:24404146 |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 35kDa |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WNT5A:301-381/381 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]. Function: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Subunit: Interacts with PORCN. Interacts with WLS. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level). Post-translational modifications: Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding. Glycosylation is necessary for secretion but not for activity. DISEASE: Defects in WNT5A are the cause of Robinow syndrome autosomal dominant (DRS) [MIM:180700]. A disease characterized by short-limb dwarfism, costovertebral segmentation defects and abnormalities of the head, face and external genitalia. The clinical signs are generally milder in dominant cases. Similarity: Belongs to the Wnt family. SWISS: P41221 Gene ID: 7474 Database links: Entrez Gene: 7474 Human Entrez Gene: 530005 Cow Entrez Gene: 22418 Mouse Entrez Gene: 64566 Rat Omim: 164975 Human SwissProt: P41221 Human SwissProt: P22725 Mouse SwissProt: Q5PY99 Rat SwissProt: Q9QXQ7 Rat Unigene: 643085 Human Unigene: 287544 Mouse Unigene: 48749 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. WNT5A蛋白属于Wnt原癌基因家族中的一种。Wnt5a与肿瘤、发生、转移有关。 |
| 产品图片 |  Sample:Uters(Mouse) Lysate at 30 ug Primary: Anti-WNT5A (bs-1948R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 47 kD  Sample:Ovary(Mouse) Lysate at 30 ug Primary: Anti-WNT5A (bs-1948R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 47 kD  Images provided the Independent Validation Program (badge number 029641)Formalin-fixed and paraffin embedded human breast labeled with Rabbit Anti-WNT5A Polyclonal Antibody (bs-1948R) at 1:250 at room temperature overnight followed by conjugation to secondary antibody.  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-WNT5A Polyclonal Antibody, Unconjugated(bs-1948R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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文献和实验Regulation of Focal Adhesion Dynamics by Wnt5a Signaling
Wnt5a is a representative ligand that activates the β-catenin-independent pathway of Wnt signaling in mammals. This pathway might be related to planar cell polarity signaling in Drosophila . Because reliable biochemical assays to measure Wnt5a
Cancer Cell：中山大学刘卓炜团队揭示膀胱癌成纤维细胞新亚群介导肿瘤干性和化疗抵抗
Promote Cancer Stemness via WNT5A in Bladder Cancer 的研究成果，该研究在膀胱癌组织中首次鉴定出一类高表达尿素转运子（SLC14A1）的成纤维细胞新亚群。证实了干扰素信号可以诱导这一亚群的分化，并通过分泌 WNT5A 活化肿瘤细胞 β-catenin 蛋白及其下游通路，从而增强肿瘤干性和介导化疗抵抗。研究人员通过单细胞测序对膀胱癌患者的肿瘤组织和配对癌旁组织中的成纤维细胞进行了无监督聚类。除了常见的 iCAF 和 mCAF 外，还发现了一类 SLC
。HSP以直接或间接的方式参与了细胞凋亡的调节，其作用靶点涉及不同凋亡信号通路的各主要激酶或关键性调控蛋白。除对凋亡 相关信号转导通路的蛋白质直接调节作用之外，还涉及了一些细胞膜生长因子受体，如EGFR、ErbB2、FLT3及细胞内雌、雄激素受体等蛋白质稳定性和 活性的调节，这种调节作用具有双向性。即既有抗凋亡的作用，也有促凋亡的作用。细胞凋亡分为线粒体通路介导的凋亡及死亡受体通路介导的凋亡，而HSP不同 程度地对这两条通路的活化具有调节作用。Hsp90能过阴断Apaf-1寡聚化，最终阴断凋亡体的形成
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