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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 靶点:
详见说明书
- 级别:
1
- 目录编号:
LM-4812R-FITC
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Anti-KLK3/Prostate Specific Antigen/FITC
- 抗体名:
Anti-KLK3/Prostate Specific Antigen/FITC
- 标记物:
FITC标记
- 宿主:
Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Guinea Pig, G
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Guinea Pig, G
- 免疫原:
详见说明书
- 亚型:
IGg
- 形态:
粉末、液体、冻干粉
- 应用范围:
ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 英文名称 | Anti-KLK3/Prostate Specific Antigen/FITC |
| 中文名称 | FITC标记的激肽释放酶3/舒血管素3抗体 |
| 别 名 | KLK3; Kallikrein 3; Kallikrein-3; APS; Gamma seminoprotein; hK3; Kallikrein related peptidase 3; KLK 3; KLK-3; KLK2A1; KLK3; P 30 antigen; P30 antigen; Prostate Specific Antigen; PSA; Semenogelase; Seminin; KLK3_HUMAN; Prostate-specific antigen; P-30 antigen. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 心血管 细胞生物 信号转导 激酶和磷酸酶 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Guinea Pig, G |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 23kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | manKLH conjugated synthetic peptide derived from human Prostate Specific Antigen |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Function: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Subunit: Forms heterodimer with SERPINA5. Subcellular Location: Secreted. Tissue Specificity: Prostate. Similarity: Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain. Database links: Entrez Gene: 354 Human Omim: 176820 Human SwissProt: P07288 Human Unigene: 171995 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. KLK3目前认为是前列腺癌很有意义的特异性标志物。激肽释放酶KLK是激肽系统的主要限速酶,它是一组存在于多数组织和体液中的丝氨酸蛋白酶,是一种肽链内切酶。KLK又称血管舒缓素,包括15个家族成员。在不同的组织中广泛表达,具有蛋白水解酶的活性。它特异性的在碳末端切割底物肽,可裂解激肽原释放具有活性的激肽,由激肽发挥对心血管系统及肾脏功能的调节作用。 组织KLK是一个大的基因家族,主要分布在肺、肾、血管、脑、肾上腺组织,为一种中等大小的糖蛋白 |
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文献和实验-8) , 再与 HRP 标记的激肽释放酶 8(KLK-8) 抗体结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的激肽释放酶 8(KLK-8) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中人激肽释放酶 8(KLK-8) 浓度。 试剂盒组成 : 试剂盒组成
抗大鼠波形蛋白单克隆抗体,l:200稀释度),4℃过夜或37℃孵育30分钟,BS漂洗3次,每次5分钟; (7)滴加与第一抗体种属匹配的荧光素标记二抗(如羊抗小鼠FITC-IgG。1:100),37℃避光孵育30分钟,PBS漂洗3次,每次5分钟; (8)Hoechst 333442复染细胞核15~20分钟(不是必需步骤); (9)甘油磷酸缓冲液封片,荧光显微镜下镜观察; (10)结果分析:波形蛋白免疫反应阳性细胞胞质呈现绿色荧光,如细胞核复染后可呈现亮蓝色荧光。阴性细胞仅见细胞
-1) , 再与 HRP 标记的激肽释放酶 1(KLK-1) 抗体结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的激肽释放酶 1(KLK-1) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中人激肽释放酶 1(KLK-1) 浓度。 试剂盒组成 : 试剂盒组成
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