Product Description: Affinity purified rabbit polyclonal phospho-serine antibodies are developed using a phospho-serine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-serine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phospho-serine antibodies from another source.
Species: Rabbit
Formulation: 250 µg/mL in PBS, 50% glycerol
Immunogen: Phospho-serine KLH and phosvitin mixture
Purification: Affinity purified with phospho-tyrosine on agarose
Specificity :The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine.
Tested :Human breast cancer tissue and mouse cell
Applications: ELISA (1:250); WB (1:250); IP (10 µg/mg crude protein); IHC (1:50)
Scientific Description :Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes.
Storage & Stability: Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
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